源于节节麦的高抗条锈小麦新材料的醇溶蛋白带谱分析  

Seed gliadins analysis of 11 stripe rust resistant lines derived from direct hybridization of common wheat with Aegilops tauschii

作　　者：杨武云[1] 陆春明[2] 卢宝荣[2] 王宇[1] 胡晓蓉[1] 余毅[1] 张顒[1] 

机构地区：[1]四川省农业科学院作物研究所,四川成都610066 [2]复旦大学生物多样性科学研究所生物多样性科学和生态工程教育部重点实验室,上海200433

出　　处：《西南农业学报》2003年第4期4-7,共4页Southwest China Journal of Agricultural Sciences

基　　金：中国博士后基金(2002031179);国家自然科学基金(30070472);四川省跨世纪杰出青年基金;四川省"十五"作物育种攻关课题资助

摘　　要：利用高抗条锈节节麦野生资源SQ 214与普通小麦直接杂交转育,培育出11份高抗条锈的小麦新材料。经A PAGE分析,节节麦SQ 214的醇溶蛋白Gli Dt1编码基因已转育到育成的高抗条锈新材料中并正常表达,其中1份高代系缺乏Gli D1位点编码的ω区醇溶蛋白带,为Gli D1"缺失体"。本文还对抗条锈高代系在抗病和品质育种中的利用价值进行了讨论。One Aegilops tauschii accession(SQ-214)from CIMMYT was found to be immune to or highly resistant to Chinese new stripe rust races CYR30 and CYR31 at adult stage.To exploit the resistance of SQ-214 for common wheat breeding,SQ-214 was crossed with Chinese Spring and backcrossed with SW3243,and eleven advanced lines with high level of resistance were developed.The gene of Gli-D1 from SQ-214 encoding Gliadins was transferred and expressed in advanced lines revealed by A-PAGE.One of advanced lines possesses a null Gli-D1 allele,of which the omega-gliadin bands encoded by the Gli-D1 allele were absent.The potential utilization of this advanced line for wheat quality and stripe rust resistance breeding was also discussed in this paper.

关 键 词：节节麦 杂交转育 小麦 高抗条锈 SQ-214 醇溶蛋自 抗病 品质 育种 利用价值 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]