组织工程技术体外重建角膜上皮层的实验研究  

Experimental study on reconstruction of corneal epithelium in vitro using tissue engineering technique

作　　者：傅瑶[1] 范先群[1] 胡晓洁[1] 刘伟[1] 崔磊[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院眼科

出　　处：《中国实用眼科杂志》2004年第1期40-43,共4页Chinese Journal of Practical Ophthalmology

基　　金："国家重点基础研究发展规则"项目 (973);组织工程的基本科学问题 (编号 :G1 9990 5430 9)资助

摘　　要：目的　利用组织工程技术体外重建角膜上皮组织 ,为眼表重建提供良好的移植材料及方法。方法　以去除上皮细胞的羊膜基底膜为载体 ,分别种植兔角膜缘组织块及原代培养扩增的角膜缘上皮细胞 ,体外培养重建角膜上皮层 ,并进行形态、组织学、超微结构观察及免疫细胞化学检测。结果　角膜缘上皮细胞在羊膜上粘附生长并增殖 ,体外培养 10天左右即形成 3～ 4层上皮细胞 ,复合角膜上皮组织由羊膜基底膜和复层上皮细胞组成 ,细胞表达特异性角蛋白CK3 ,与生理状态下的角膜上皮组织相近。结论　以羊膜为载体培养角膜缘上皮细胞可体外重建组织工程化角膜上皮组织 ,作为眼表重建移植材料良好的来源。Objective To reconstruct corneal epithelium in vitro by tissue engineering technique and provide effective method and materials for clinical transplantation.Methods Small limbal tissues or primary cultured limbal epithelial cells were seeded ont he basement membrane of amnion which had been prepared by removing of the original epithelium,the composite corneal epithelium were cultured in vitro and then the reconstructed tissues were examined in histological sructure,ultrastructure and immunocytochemical behavior.Results Limbal epithelial cells can be cultured and proliferated on the amniotic membrane.3～4 layers of stratified epithelium was formed after 10 days.The composite epithelium was composed of the basement membrane and stratified epithelial cells,which expressed corneal epithelial cytokeratin(CK3),the cultured tissue proved to resemble the normal epithelial layer.Conclusions Tissue engineered corneal epithelium can be reconstructed in vitro by culturing limbal epithelial cells on aminiotic membrane,which provides quality material for ocular transplantation.

关 键 词：组织工程 体外 角膜上皮层重建 实验 形态学 组织学 超微结构 

分 类 号：R318.18[医药卫生—生物医学工程] R-33[医药卫生—基础医学]