骨髓基质细胞的血管新生作用及对心功能的影响  

The neovascularization in ischemic myocardium by bone marrow stromal cells transplanting and its impact on cardiac function

作　　者：蒋世忠[1] 黄岚[1] 武晓静[1] 于世勇[1] 宋明宝[1] 张坡[1] 赵刚[1] 晋军[1] 周音频[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,重庆市400037

出　　处：《岭南心血管病杂志》2003年第6期426-429,共4页South China Journal of Cardiovascular Diseases

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 5 68)

摘　　要：目的　观察骨髓基质细胞在缺血心肌内诱导血管新生的作用及对心功能的影响。方法　复制兔心肌梗死动物模型 ,3d后将体外分离扩增的自体骨髓基质细胞用荧光标记物DAPI标记后移植到梗死周围缺血区心肌 (细胞移植组 ,n =8) ,对照组注射等量培养基 (n =6 )。 4W后 ,先进行心脏超声检查分别测定对照组和细胞移植组左室射血分数 (EF)和短轴缩短率 (FS) ,然后采集移植区心肌标本 ,用荧光示踪方法观察移植细胞在缺血坏死区心肌存活的情况 ,用CD31单克隆抗体免疫组化染色法测定毛细血管密度。结果　细胞移植组心肌组织和小血管内壁均可见蓝色荧光的DAPI标记的移植细胞 ;细胞移植组心肌毛细血管密度显著高于对照组 [(16 3 0 0± 2 5 85 )vs (96 0 0± 16 6 1) ,P <0 0 5 ];细胞移植组EF较对照组增高 [(0 5 0± 0 0 3)vs(0 4 6± 0 0 3) ,P <0 0 5 ],短轴缩短率FS也高于对照组[(2 7 0 2± 1 2 7)vs (2 3 85± 1 6 9) ,P <0 0 5 ]。结论　体外扩增的骨髓基质细胞可在缺心肌内存活 ,并能促进缺血组织血管新生、改善心功能。Objectives To investigate the neovascularization of ex vivo expanded bone marrow stromal cells (MSCs) in ischemic myocardium and its effects on cardiac function. Methods Myocardial infarction was induced by ligating the left anterior descending coronary artery of the rabbits. Three days later, the isolated and cultured MSCs were labeled with the fluorescence tracer DAPI, and then transplanted respectively into the ischemic myocardium of itself (transplantation group, n=8), other rabbits received equal culture media (control group, n=6). 4 weeks later, the echocardiography was performed to analysis the left ventricular ejection fractions (EF) and fraction shorting (FS). Then, the myocardium was harvested. Fluorescence tracing was used to determine the survival of the transplanted MSCs in the ischemic myocardium. Immunohistochemical staining was used to analysis the capillary density. Results The implanted MSCs were found in the ischemic tissue and within the wall of small vessels in the area adjacent to myocardial infarction by fluorescence microscope; The capillary density was significantly greater in MSCs group than that in the control group [(163.00±25.85) vs (96.00±16.61),P<0.05]; In the control group, the capillary were found only in the ischemic area, however in the transplantation group, the capillary were found both in the ischemic and the infarct area. The EF and FS in the transplantation group was higher than that in the control group EF[(0.50±0.03) vs (0.46±0.03), P<0.05]; FS[(27.02±1.27) vs (23.85±1.69), P<0.05]. Conclusions Ex vivo expanded MSCs can promote neovascularization of the ischemic myocardial and improve cardiac function.

关 键 词：骨髓基质细胞 血管 心功能 细胞移植 

分 类 号：R541[医药卫生—心血管疾病]