HPLC测定己酮可可碱氯化钠注射液中主药及有关物质  

Determination of pentoxifylline and its related substances in Pentoxifylline and Sodium chloride injection by HPLC

作　　者：于丽芬[1] 钟琼[1] 葛求富[1] 

机构地区：[1]杭州民生药业集团有限公司,浙江杭州310011

出　　处：《华西药学杂志》2003年第6期450-451,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的　建立己酮可可碱氯化钠注射液中己酮可可碱的测定方法。方法　色谱柱用HypersilBDSC18柱 (15 0mm× 4 .6mm ,5 μm) ;流动相为甲醇 -水 (5 5∶4 5 ) ;检测波长 2 74nm ;流速 1.0ml·min-1;进样量 2 0 μl。结果　己酮可可碱在浓度 1.0～ 6 0μg .ml-1范围内线性关系良好 (r=0 .9999) ,平均回收率为 99.8% ,RSD =0 .4 % ,有关物质限量在 0 .75 %～ 2 .5 %内 ,自身对照主成分峰面积与浓度呈良好的线性关系。结论　所用方法专属性强 ,且简便、灵敏、准确。OBJECTIVE To establish an HPLC method to determining pentoxifylline and its related substances in Pentoxifylline and Sodium chloride injection.METHODS A column packed with BDS C 18 was used.The mobile phase was methanol-water (55∶45) and the flow rate was 1.0 ml·min -1.The UV detection wavelength was of at 274 nm.RESULTS The calibration curve was linear in the range of 1.0 μg·ml -1 to 60.0 μg·ml -1 (r=0.9999).The average recovery was 99.8% with RSD of 0.4%.In the range of 0.75%-2.5%, the related substances has good linearity.CONCLUSION The method is selective,simple and accurate.

关 键 词：HPLC 测定 己酮可可碱 氯化钠注射液 主药 

分 类 号：R927.2[医药卫生—药学]