HPLC测定人血清中利福平的浓度  

Determination of Rifampicin in human serum by HPLC

作　　者：刘荣[1] 张显杰[1] 王仲元解放军第309医院结核科 陈红兵解放军第309医院结核科 魏荣[1] 刘洁[1] 刘蔚[2] 胡麟[1] 

机构地区：[1]解放军第309医院药剂科,北京100091 [2]解放军第309医院药理科,北京100091

出　　处：《华西药学杂志》2003年第6期446-448,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的　建立测定血清中利福平浓度的高效液相色谱法 ,并应用于临床。方法　采用Nova -pakC18色谱柱 (4 μm ,15 0nm× 3.9mm) ,以甲醇 - 0 .0 2mol·L-1醋酸钠 (6 2∶4 0 )为流动相 ,紫外检测波长 334nm ,测定口服利福平 4d以上的患者血清中利福平的浓度。结果　血清中利福平在 0 .5～ 16 .0 μg·ml-1范围内浓度与峰面积线性关系良好 (r=0 .9992 ) ,平均回收率为99 .4 3% ,日内、日间RSD <5 .0 %。结论　所用方法准确、灵敏 ,用血量少 ,适用于治疗药物监测 。OBJECTIVE To establish a method to determine the Rifampicin concentration in human serum by HPLC for clinical application.METHODS The determination of Rifampicin was carried out on C 18 Nova-pak column with the mobile phase of CH 3OH-0.02 mol·L -1NaAc(62∶40).The UV detective wavelength was at 334 nm.The method has been used to determine Rifampicin in serum after patients took oral dosage drugs more than 4 days.RESULTS A good linearity was obtained from 0.5-16.0 μg·ml -1 with a correlation coefficient of 0.9992.The average recovery of the assay was 99.43%.The RSD of inter-day and intra-day were less than 5.0%(n=6).CONCLUSION The method appeared to be accurate and sensitive. It can be used for monitoring Rifampicin concentrations in serum and provided reference data for rational drug usage in clinic.

关 键 词：HPLC 测定 人血清 利福平 浓度 

分 类 号：R969.1[医药卫生—药理学]