RGD肽对内皮细胞在聚酯材料表面粘附、增殖的影响  

Effect of RGD Peptides on Endothelial Cell Adhesion and Proliferation on Polyethylene Terephthalate Surfaces.

作　　者：武忠[1] 石应康[2] 赁可[2] 

机构地区：[1]南京大学医学院附属鼓楼医院胸心外科,210008 [2]四川大学华西医院胸心外科,610041

出　　处：《北京生物医学工程》2003年第4期278-280,共3页Beijing Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 2 98740 2 2 )

摘　　要：RGD是许多粘附蛋白结构中的高度保守序列 ,与细胞在生物材料表面的粘附、增殖密切相关。本研究在聚酯薄膜表面分别预衬纤维粘连蛋白和共价接枝RGD三肽 ,然后在不同聚酯材料上种植体外培养的人脐静脉内皮细胞 ,结果显示RGD可明显促进细胞在材料表面的粘附和增殖 ,与纤维粘连蛋白相比 ,RGD促进细胞粘附的作用更为明显 ,而在细胞增殖方面 ,二者的作用无显著性差异。本研究为改进生物材料的表面设计 。Many kinds of adhesive proteins contain Arg Gly Asp (RGD) sequence, which is closely related to cell adhesion and proliferation on biomaterial surfaces. In this study,cultured human umbilical vein endothelial cells (HUVEC) were seeded on polyethylene terephthalate (PET) surfaces, which were precoated with fibronectin, or covalently grafted with synthetic bioactive RGD peptides respectively. The results showed that synthetic RGD peptides could remarkably improve cell attachment and proliferation on PET surfaces. Compared with fibronectin, RGD showed more significant cell adhesion activity, while there was no obvious difference of proliferation activity between fibronectin and RGD peptide. This study provides a feasible idea of biomaterial surface modification, and may improve clinical results of endothelialized biomaterials.

关 键 词：RGD肽 内皮细胞 聚酯材料 细胞粘附 细胞增殖 生物材料 心血管移植物 

分 类 号：R318.08[医药卫生—生物医学工程]