Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope  

作　　者：LIHui CHENDeliang 等 

机构地区：[1]StateKeyLaboratoryofMagnetism,InstituteofPhysics,CenterofCondenseMatterPhysics,ChineseAcademyofSciences,Beijing100080,China [2]InstituteofBiophysics,ChineseAcademyofSciences,Beijing

出　　处：《Chinese Science Bulletin》2003年第6期573-576,共4页

基　　金：supported by the State Key Basic Research and Development Plan Project(Grant No.199801012).

摘　　要：Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were com- pared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hex- agonal structure. We assume that it was caused by the inter- action between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR抯 biochemical functions have been changed.Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were com- pared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hex- agonal structure. We assume that it was caused by the inter- action between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR抯 biochemical functions have been changed.

关 键 词：嗜盐细菌 紫色细胞膜 CHAPS 洗涤剂 表面结构 原子力显微镜 

分 类 号：Q934[生物学—微生物学] Q735