B→O blood conversion  

作　　者：ZGHANYangpei LANJiongcai 等 

机构地区：[1]InstituteofBloodTransfrusion,AcademyofMilitaryMedicalSciences,Beijing100850,China [2]SouthHospital,TheFirstMilitaryMedicalUniversity,Guangzhou510515,China

出　　处：《Chinese Science Bulletin》2003年第3期269-273,共5页

基　　金：supported by the State"863"High-tech Project(Grant No.102-09-04-02);the National Key Basic Research and Development Program(Grant No.2002CB713804);the PLA Ten-Five Key Research Program(Grant No.2000252910).

摘　　要：To maintain a constant supply of universal blood type, or group O red blood cells, has benefit in specialized transfusion condition. In this study, a-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for a-galactosidase cDNA expression and transferred a-galactosidase cDNA into Pichia pastoris GS115 cells by elec-troporation. The recombinant a-galactosidase (r-a-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-a-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe.To maintain a constant supply of universal blood type, or group O red blood cells, has benefit in specialized transfusion condition. In this study, a-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for a-galactosidase cDNA expression and transferred a-galactosidase cDNA into Pichia pastoris GS115 cells by elec-troporation. The recombinant a-galactosidase (r-a-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-a-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe.

关 键 词：红细胞血型 B型 血型转化 O型 重组α-半乳糖苷酶 

分 类 号：R457.11[医药卫生—治疗学]