Comparative analysis of the three-dimensional structure of Periplaneta fuliginosa densovirus  

作　　者：LILi CHENDonghua 等 

机构地区：[1]InstituteofVirology,WuhanUniversity,Wuhan430072,China [2]BeijingLaboratoryofElectronMicroscopy,ChineseAcademyofSciences,Beijing100080,China

出　　处：《Chinese Science Bulletin》2003年第3期277-281,共5页

基　　金：supporled by the National Natural Science Foundation of China(Grant No.39470032)

摘　　要：The three-dimensional structure of Periplaneta fuliginosa densovirus (pfDNV) is determined at 2.3 nm reso-lution using the techniques of cryo-electron microscopy and image reconstruction. The pfDNV contains five structural proteins and 60 protein subunits arranged on a T = 1 icosa-hedral shell with a relatively smooth surface. Its recon-struction reveals its distinct capsid structure from those ob-served in CPV and GmDNV. As in GmDNV, spike-like protrusions are not present in pfDNV at the threefold axes; while two small thorn-like protrusions are identified there. However, different from CPV and GmDNV, cylindrical channels along the fivefold axes are closed in pfDNV; while a small thorn-like protrusions, which have not been reported in other parvovirus, are observed there in pfDNV although their function is yet to be investigated. The pfDNV has dim-ple-like depressions at the icosahedral twofold axes; but has no canyon-like regions encircling the fivefold axes. The ico-sahedrally well-ordered nucleic acid has also been observed in pfDNV, suggesting that the protein and nucleic acid probably form closed interaction.The three-dimensional structure of Periplaneta fuliginosa densovirus (pfDNV) is determined at 2.3 nm resolution using the techniques of cryo-electron microscopy and image reconstruction. The pfDNV contains five structural proteins and 60 protein subunits arranged on a T = 1 icosahedral shell with a relatively smooth surface. Its reconstruction reveals its distinct capsid structure from those observed in CPV and GmDNV. As in GmDNV, spike-like protrusions are not present in pfDNV at the threefold axes; while two small thorn-like protrusions are identified there. However, different from CPV and GmDNV, cylindrical channels along the fivefold axes are closed in pfDNV; while a small thorn-like protrusions, which have not been reported in other parvovirus, are observed there in pfDNV although their function is yet to be investigated. The pfDNV has dimple-like depressions at the icosahedral twofold axes; but has no canyon-like regions encircling the fivefold axes. The icosahedrally well-ordered nucleic acid has also been observed in pfDNV, suggesting that the protein and nucleic acid probably form closed interaction.

关 键 词：蟑螂病毒 pfDNN 昆虫细小病毒 三维结构 比较研究 电镜 

分 类 号：R373.3[医药卫生—病原生物学] Q939.4[医药卫生—基础医学]