地甘口服液对辐射损伤小鼠细胞周期及凋亡相关基因的影响  

Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions

作　　者：何东初[1] 王晓红[1] 何辉[1] 陈如泉[2] 

机构地区：[1]广州军区武汉总医院中西医结合科,武汉430070 [2]湖北省中医学院附院

出　　处：《中华放射医学与防护杂志》2003年第6期432-434,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：湖北省自然科学基金资助项目( 97J080 )

摘　　要：目的　探讨中药复方地甘口服液对辐射损伤小鼠骨髓细胞周期素基因 (cyclinD1)及凋亡相关基因 (bcl 2和bax)mRNA表达的作用。方法　建立辐射损伤模型 (一次性用直线加速器给予6 0Gy的X射线照射 ) ,喂饲小剂量 10 0 %和大剂量 2 0 0 %的地甘口服液每次 0 2ml 10g小鼠 ,每日 2次。第 9天用原位杂交法检测小鼠骨髓单个核细胞cyclinD1、bcl 2和baxmRNA的表达。结果　地甘口服液组与对照组比骨髓细胞促凋亡基因baxmRNA表达显著减少 (P <0 0 1) ,而cyclinD1和bcl 2m RNA的表达显著增强 (P <0 0 1)。结论　中药复方地甘口服液抗辐射损伤可能是通过上调细胞周期素基因cyclinD1和bcl 2的表达及降低baxmRNA的表达 ,从而促进造血细胞的增生和提高机体免疫力 ,达到保护机体的目的 。Objective To study the effect of Digan oral liquid (DGOL) on expression of cell cycle prog ression(cyclinD-1)- and apoptosis(bcl-2 and bax)- related genes in bone marr ow of mice with radiation lesions. Methods After the radiation lesion model(given 6 0 Gy X-rays on ce by a linear accelerator) was established,the animals were fed with 0 2 ml/10 g of either small dose (100%) or large dose (200%) DGOL twice a day for 8 days. On the ninth day cyclinD-1,bcl-2 and bax mRNA expression levels in marrow MNC of model mice were measured by in situ hybridization. Results In the DGOL group,the bax mRNA expression was significantly decreased (P<0 01),but cyclinD-1 and bcl-2 mRNA were significantly increased (P<0 01) t han those in the control group. Conclusion DGOL can restrain radiation lesion due to its enhancing effects on cyclinD-1 an d bcl-2 mRNA expression,and inhibitory effect on bax mRNA expression.In this wa y,it prevents the blood cells from injury,promotes immunity,and protects the org anism.This result provides a theoretical basis for clinical application of Diga noral liquid.;

关 键 词：地甘口服液 辐射损伤 凋亡 小鼠 细胞 

分 类 号：R96[医药卫生—药理学]