中国慢性疲劳综合征患者血浆中BDV-p24抗体的检测  

Detection of antibody against Borna disease virus-p24 in the plasma of Chinese patients with chronic fatigue syndrome by Western-blot analysis

作　　者：李永杰[1] 王得新[2] 张凤民[3] 刘占东[2] 杨爱英[3] 生田和良[4] 

机构地区：[1]首都医科大学附属北京友谊医院神经内科北京京煤集团总医院神经内科,100050 [2]首都医科大学附属北京友谊医院神经内科,100050 [3]哈尔滨医科大学微生物学教研室 [4]日本大阪大学微生物病研究所

出　　处：《中华实验和临床病毒学杂志》2003年第4期330-333,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：本课题由国家自然科学基金资助(30070673)

摘　　要：目的检测博尔纳病病毒(BDV)对中国慢性疲劳综合征(CFS)患者和健康对照者感染的情况,探讨CFS与BDV感染之间的相关性。方法按美国CDC 1994年标准,搜集来自全国十一省市的CFS患者61例和健康对照73例,使用蛋白印迹法(WB)对其血浆进行BDV-p24抗体检测。结果 病例组有7例为阳性结果(11.48％),对照组均为阴性(0％),统计学处理两组间差异有显著意义(P<0.010)。结论中国CFS患者存在BDV感染,病例组感染率明显高于对照组,CFS与BDV感染存在相关性。Objective To evaluate the prevalence of infection with Borna disease virus (BDV) in Chinese patients with chronic fatigue syndrome (CFS) and control subjects, and to discuss the etiological association between CFS and infection with BDV. Methods The CDC (1994) diagnostic criteria for CFS were used for case definition. Sixty-one patients suffered from CFS were from 11 Provinces in China. To detect the antibody against BDV-p24 on the plasma samples from all cases and 73 healthy control subjects by Western-blotting analysis. Results 7 of the Sixty-one cases and 0 of the controls were sero-positive for BDV-p24 antibody, there was a statistical significant difference between the two groups (11.48% vs 0% ; P < 0.010) . Conclusion Chinese patients with CFS showed sero-positive identifying BDV infection, by comparison, anti-BDV-p24 antibody prevalence in patients was significantly higher than in controls. An etiological association may exist between CFS and BDV infection.

关 键 词：中国慢性疲劳综合征 博尔纳病病毒 BDV CFS 病因学 抗原 血清 

分 类 号：R446.62[医药卫生—诊断学]