巨细胞病毒感染在2型糖尿病发病中作用的研究  

Rote of cytomeggalovirus infection in the pathogenesis of type 2 diabetes mellitus

作　　者：梁浩[1] 梁瑜祯[2] 陈晖[2] 于志清[2] 苏洁寒[1] 邬质彬[1] 覃锦耀[2] 

机构地区：[1]广西医科大学公共卫生学院,南宁530021 [2]广西医科大学第一附属医院

出　　处：《中华实验和临床病毒学杂志》2003年第4期351-353,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：广西教育厅课题项目(19982-9)

摘　　要：目的从定量水平上深入研究人类巨细胞病毒(HCMV)感染在2型糖尿病发病中的作用。方法采用定量PCR技术测量29例2型糖尿病患者和23例对照组血清中HCMV DNA含量,并与外周血T细胞亚群、血糖(BG)、胰岛素(Ins)和C肽(C-P)水平进行分析。结果 2型糖尿病患者HCMV DNA含量[(1.81±1.67)×108拷贝/ml]明显高于对照组[(5.50±4.30)×107拷贝/ml]。HCMVDNA阳性患者CD8百分率(29.53％±2.00％)显著高于对照组(27.13％±4.12％),CD4/CD8比值(1.24±0.05)显著低于对照组(1.41±0.10)。HCMV DNA阳性患者与阴性患者比较,空腹C-P明显降低,而BG和Ins差异无显著意义。结论2型糖尿病患者HCMV感染的活化,可影响细胞免疫,在2型糖尿病发病中的作用值得进一步研究。Objective To further study the role of human cytomegalovirus ( HCMV) infection in the pathogenesis of the type 2 diabetes mellitus. Methods HCMV DNA levels in sera from 29 type 2 diabetic patients and 23 controls were measured by quantitative polymerase chain reaction (PCR), and comparative analyses was made between HCMV DNA and T cell subsets, blood glucose (BG), insulin (Ins) and C peptide (C-P). Results The levels of HCMV DNA were (1.81±1.67)×108 copies/ml for type 2 diabetic patients, a level significantly higher than that [(5.50±4.30)×107 copies/ml] of controls. The percentage of CD8 for type 2 diabetic patients with positive HCMV DNA was 29.53%±2.00%,being much higher than that for controls (27.13%±4.12% ), while the ratio of CD4/CD8 (1.24±0.05)was significantly lower than that( 1.41 ±0.10) of controls. Fasting C-P of type 2 diabetic patients with positive HCMV DNA was far lower than that of those with negative HCMV DNA, but the differences of BG and Ins between the two groups were not significant. Conclusion Active HCMV infection of type 2 diabetic patients may suppress cellular immunity and it' s influence on the pathogenesis of the type 2 diabetes mellitus should be further studied.

关 键 词：巨细胞病毒 病毒感染 2型糖尿病 HCMV T淋巴细胞亚群 聚合酶链反应 非胰岛素依赖型 

分 类 号：R587.1[医药卫生—内分泌] R511[医药卫生—内科学]