戊二醛偶联组氨酸修饰金电极测定铜离子的研究  

Glutaraldehyde Modified Gold Electrode for Subsequent Histidine Attachment and Determination of Copper

作　　者：李昌安[1] 葛存旺[1,2] 刘战辉[1] 张海黔[1] 顾宁[1] 

机构地区：[1]东南大学纳米科学与技术中心,分子与生物分子电子学教育部重点实验室,南京210096 [2]南通师范学院化学系,江苏南通226007

出　　处：《传感技术学报》2003年第4期475-479,共5页Chinese Journal of Sensors and Actuators

摘　　要：本文主要研究了一种基于戊二醛偶联组氨酸修饰金电极的方法 ,并以该修饰电极为工作电极利用方波伏安法建立了一种检测痕量铜离子的新方法。在铜溶液中搅拌富集 ,铜离子与修饰电极表面的组氨酸形成配合物吸附在电极表面 ,在磷酸缓冲液 (pH 6 8)中 ,该配合物具有良好的电化学响应。在对不同浓度的铜离子进行检测时发现在 1× 10 - 1 2 ～ 1 8× 10 - 1 1 mol/L和 5× 10 - 7～ 2 1× 10 - 5mol/L之间方波伏安还原峰电流与铜离子浓度呈现良好的线性关系 ,其最低检测限可达 0 5× 10 - 1 2 mol/L 。A new chemically modified gold electrode was prepared by Glutaralhehyde modified electrode for subsequent histidine attachment. A new approach was developed for sensitive and selective determination of copper by the electrode. A complex of copper with the modifier was formed and accumulated on surface of the electrode while immersed the electrode into a copper-containing solution. In a NaH 2PO 4-Na 2HPO 4buffer solution (pH6.8), the electrode has good electro activity. While adding copper,the current increase and the potential remains same. The value of peak current is linear with the copper concentration in ranges 1×10 -12～1.8×10 -11 mol/L and 5×10 -7～2.1×10 -5 mol/L. The detection limits is 0.5 pmol/L and an indirect mechanism of determination is proposed.

关 键 词：戊二醛 组氨酸 修饰金电极 铜离子检测 电化学传感器 偶联剂 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置] O614.121[自动化与计算机技术—控制科学与工程]