雷洛昔芬对血管内皮素-1基因mRNA表达的影响及与雌激素受体的关系  

The effect of selective estrogen receptor modulator,raloxifene on the gene expression of endothelin-1 in vascular endothelial cells and the relationship with estrogen receptor

作　　者：贺红[1] 杨发林[2] 朱清[1] 吴逸南[1] 刘贤锡[3] 王树春[4] 

机构地区：[1]山东大学齐鲁医院心内科,济南市250012 [2]山东大学齐鲁医院检验科,济南市250012 [3]山东大学分子生物学教研室 [4]山东大学第二附属医院内科

出　　处：《中华老年医学杂志》2003年第12期747-749,共3页Chinese Journal of Geriatrics

摘　　要：目的　观察选择性雌激素受体调节剂雷洛昔芬 (Raloxifene)对血管内皮细胞内皮素 1基因mRNA表达的影响 ,并探讨Raloxifene的作用是否通过雌激素受体机制介导。　方法　采用Raloxifene处理培养的牛颈动脉内皮细胞 2 4h ,然后提取细胞总RNA ,取 2 0ug进行Northernblotting分析 ,观察Raloxifene对血管内皮细胞内皮素 1基因mRNA表达的影响 ,同时采用雌激素受体阻断剂ICI182 780处理细胞 ,观察内皮素 1基因表达的变化。　结果　与未经Raloxifene处理的细胞相比 ,Raloxifene处理后的血管内皮细胞内皮素 1基因mRNA表达明显降低〔分别为 (0 16± 0 0 5 ) ,(0 39± 0 0 7) ,P <0 0 5〕若同时应用ICI182 780处理 ,内皮素 1mRNA表达增强 (0 6 9± 0 0 8,P <0 0 5 ) ,Raloxifene的作用被阻断。　结论　选择性雌激素受体调节剂Raloxifene通过雌激素受体机制抑制血管内皮细胞内皮素 1基因表达。Objective To investigate the effect of raloxifene on the mRNA expression of endothelin-1 in vascular endothelial cells and assess the role of estrogen receptor. Methods Bovine carotid endothelial cells were pretreated with 10nM raloxifene for 24 hours, then total RNA was harvested and Northern blotting was performed to investigate the effect of raloxifene on the mRNA expression of endothelin-1. Furthermore, estrogen receptor inhibitor, 100nM ICI 182 780 was used to pretreat the cells together with raloxifene and the expression of endothelin-1 was observed too. Results The mRNA expression of endothelin-1 in bovine carotid endothelial cells was inhibited significantly by pretreatment with raloxifene and this effect could be blocked by ICI182 780 (0.16±0.05 vs 0.39±0.07, P <0.05). Conclusions The selective estrogen receptor modulator, raloxifene may inhibit the gene expression of endothelin-1 in vascular endothelial cells via estrogen receptor.

关 键 词：雷洛昔芬 血管 雌激素受体调节剂 雷洛昔芬 内皮细胞 受体 冠心病 

分 类 号：R96[医药卫生—药理学]