植物蛋白MAP30体外抗HIV-1的实验研究  

IN VITRO EVALUATION ON THE EFFECTS OF PLANT PROTEIN MAP30 AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1

作　　者：王临旭[1] 孙永涛[1] 杨为松[1] 白雪帆[1] 黄长形[1] 王福祥[1] 张颖[1] 

机构地区：[1]第四军医大学唐都医院,西安710038

出　　处：《解放军医学杂志》2003年第10期894-896,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：体外观察苦瓜子抗HIV植物蛋白 30 (MAP30 )以及齐多夫足 (AZT)、阿昔洛韦 (ACV)、干扰素 α2a(IFN α2a)抗HIV 1的作用。以MT4为靶细胞 ,采用ELISA法测定药物对细胞培养上清HIV 1P2 4抗原的抑制作用 ,并观察药物对HIV 1致细胞病变效应的抑制效果。结果显示 ,MAP30具有明显的抗HIV 1作用 ,与一线抗HIV药物AZT作用相近 ,二者对HIV 1P2 4抗原的 5 0 %抑制浓度 (IC50 )分别为 0 .9μmol/L和 0 .7μmol/L ,它们对HIV 1的致细胞病变效应也有抑制作用。ACV及IFN α2a在所取浓度范围内对HIV 1无抑制作用。证实植物蛋白MAP30具有体外抑制HIV 1的作用。To evaluate the inhibitive effect of plant protein MAP30 (momordica anti-HIV protein of 30kDa), AZT (3′-azido-2′,3′-dideoxythymidine) , ACV (acyclovir) and IFN-α2a (interferon-α2a) against HIV-1 in vitro, MT4 was used as the target cell and the inhibitive effects of these drugs on HIV-1 P24 expression were investigated by ELISA. The inhibitory effect of these drugs on HIV-1-induced cytopathy was also evaluated. The 50% inhibition concentration (IC 50) of MAP30 was 0.9μmol/L. In comparison, the IC 50 for AZT , a commonly used anti-HIV drug, was 0.7μmol/L. The cytopathic effect induced by HIV-1 was also inhibited by MAP30 and AZT. ACV and IFN-α2a showed little effect on HIV-1. All these results strongly indicated that plant protein MAP30 could obviously inhibit HIV-1 replication in vitro.

关 键 词：植物蛋白质类 抗病毒药 HIV-1 齐多夫足 阿昔洛韦 实验研究 

分 类 号：R978.7[医药卫生—药品]