无细胞真皮复合皮制备及移植的实验研究  

EXPERIMENTAL STUDY ON THE PREPARATION AND TRANSPLANTATION OF COMPOSITE SKIN COMPOSED OF ACELLULAR DERMIS AND KERATINOCYTES

作　　者：刘旺[1] 肖仕初[1] 郇京宁[1] 陈玉林[1] 夏照帆[1] 

机构地区：[1]第二军医大学长海医院,上海200433

出　　处：《解放军医学杂志》2003年第10期869-870,893,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家杰出人才科学基金 (编号 3972 50 2 9);国家 973项目 (编号G1 9990 5430 9);国家 863项目 (编号 2 0 0 1AA2 1 60 4 1 );上海市卫生系统百名跨世纪优秀学科带头人培养计划 (编号 97BR0 4 6)资助课题

摘　　要：体外构建表皮细胞 无细胞真皮活性复合皮 ,移植于裸鼠背部创面 ,并与单纯移植表皮细胞膜片相对照。术后观察创面愈合情况 ,行光镜、电镜及免疫组化检测。结果示复合皮移植后创面覆盖率高 ,外观情况良好 ,收缩程度低。组织学检测提示复合皮移植后上皮分化充分 ,胶原增生有序 ,表皮 真皮连接结构重建明显 ,提示无细胞真皮复合皮移植可改善创面愈合质量。To study the method of preparing the composite skin composed of keratinocytes and acellular dermis, and to investigate the efficacy as well as its histological changes after being grafted on wounds of athymic mice. Compared with keratinocyte sheet grafting, the wounds grafted with composite skin had better healing rate, appearance and less wound contraction rate. Moreover, histological examination revealed fully differentiated epidermis, organized proliferated collagen fibres, and distinct reconstruction of epidermis-dermis junction in composite skin group, with absence of acute immuno-rejection response. As a new kind of skin substitutes, the composite skin can improve the quality of wound healing.

关 键 词：表皮细胞培养 真皮 生物敷料 皮肤移植 

分 类 号：R622.1[医药卫生—整形外科]