补虚化瘀方拮抗细胞毒剂所致荷瘤小鼠骨髓抑制的实验研究  

Effect On Bu-xu Hua-yu Prescription to Bone Marrow Depression due to Hemorrheological Indices of Carcinomatosis Mice after Chemotherapy

作　　者：勾忠平[1] 邓道昌[2] 

机构地区：[1]四川大学华西医院,四川成都610041 [2]成都中医药大学

出　　处：《成都中医药大学学报》2003年第4期37-40,65,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省中医管理局课题 (编号 :96 - 2 2 6 )

摘　　要：目的　观察补虚化瘀方对荷瘤小鼠化疗后骨抑制及血液流变学的影响。方法　对接种P388白血病瘤株的小鼠 ,当天即开始予补虚化瘀方灌胃 ,连用 1 3天。将给药小鼠分为高、低剂量组 ,另设正常、模型、化疗对照组。接种后第 6日、 7日分别给化疗、高剂量、低剂量组腹腔注射环磷酰胺(1 5 0mg/kg)、阿糖胞苷 (1 5 0mg/kg)联合冲击化疗。用流式细胞仪检测小鼠骨髓细胞增殖周期以及血液流变学的变化。结果 (1 )化疗使荷瘤小鼠骨髓细胞合成期 (S)细胞数降低 (P <0 0 1 ) ,在中药高剂量组骨髓细胞合成期 (S)细胞数升高 (P <0 0 1 ) ,并恢复至正常水平 (P >0 0 5 )。 (2 )小鼠荷瘤状态血液粘滞度增高 ,微循环障碍 ,化疗又进一步加重以上反应。中药高剂量组血液粘滞度降低、微循环得到改善 (P <0 0 5 )。结论　补虚化瘀方对环磷酰胺、阿糖胞苷所致荷瘤小鼠骨髓抑制有一定的保护作用 ;Objective:: To observe the effect of Bu-xu Hua-yu prescription (BXHYF) on bone marrows depression, hemorrheological indices of carcinomatosis mice after chemotherapy. Methods: To feed mice with BXHYF from the first day of inoculation to the thirteenthday. To devide BXHYF into two dose groups-high dose group and low dose guoup. Meanwhile three control groups were set up including normal group, carcinoma group and chemotherapy group. CTX and Ara-c(150 mg/kg) was peritoneally injected into carcinomatosis mice of high dose group, low dose group and chemotherapy group on the sixth and seventh day of inoculation respectively. To observe the influence of BXHYF on the cell cycle of bone marrows nucleated cell by flow cytometor analysis system, and on hemorheology. Result:(1) CTX and Ara-c caused obvious decreasing of the cell counts of S phase and increasing of the cell counts of G 1 phase (P<0.01). High dose group can increase the cell counts of S phase and decrease the cell counts of G1 phase. The cell counts of S phase and G 1 phase in high dose group were similar to that in normal group (P>0.05).(2)Chemotherapy exacerbate the hypercoagulability and microcirculation obstruction, which existed commonly in carcinomatosis mice. High dose group can low the degree of blood viscocity and improve microcirculation (P<0.05). Conclusion: BXHYF can protect carcinomatosis mice against bone marrows suppression by CTX and Ara-c , improve hemorrheological indices.

关 键 词：补虚化瘀方 细胞毒剂 荷瘤小鼠 骨髓 实验研究 血液流变学 细胞周期 

分 类 号：R285.5[医药卫生—中药学]