地塞米松磷酸钠脂质体经兔结肠粘膜的体外Ussing chamber渗透研究  

Ussing chamber technique for studying the permeability of dexamethasone sodium phosphate liposome through rabbit colon mucosa In vitro

作　　者：李国锋[1] 陈建海[1] 杨静[1] 郭丹[1] 任非[1] 王春霞[1] 侯连兵[1] 

机构地区：[1]第一军医大学南方医院药学部,广东广州510515

出　　处：《第一军医大学学报》2004年第1期11-14,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金(020056);广东省医学科研基金(A2002371)~~

摘　　要：目的观测并评价载药脂质体经结肠粘膜渗透的特点。方法制备地塞米松磷酸钠(DSP)脂质体,采用体外Uss-ing chamber 实验法,考察DSP经时经兔结肠粘膜的累积透过量及在粘膜内的分布量。结果和DSP溶液比较,DSP脂质体能延缓和减少DSP经结肠粘膜的透过量。DSP脂质体的表观渗透系数(×10-6,cm/s)为31.95±7.65,和DSP溶液(88.61±18.61)比较,具有显著差异。透过实验120 min和300 min时,DSP脂质体在结肠粘膜中的分布量均明显高于DSP溶液。结论和DSP溶液比较,DSP 脂质体在治疗局部结肠炎时,可能具有更小的毒性,更高的疗效,值得进一步开发研究。Objective To investigate the features of liposome-mediated dr ug permeability through the colon mucosa in vitro. Methods Dexamethasone sodium phosphate liposome (DSP) was prepared using orthogonal design. With in vitro Us sing chamber experiment, the accumulated amount of DSP permeated through rabbit colon mucosa into the receptor chamber and the distribution amount in the colo n were determined at different time points during the experiment. Results Compar ed with the prepared DSP solution, DSP liposome decreased the permeated amount of DSP through rabbit colon mucosa. The apparent permeability coefficient (Papp , ×10 -6 , cm/s1) of the DSP was 31.95±7.65, significantly lower than that of DSP so lution (88.61±18.61). On the other hand, the distribution amount of DSP liposom e in the colon mucosa was significantly higher than that of the DSP solution bo th at 120 and 300 min. Conclusion DSP liposome dosage form may induce less toxic ity with better thera-peutic effect in the treatment of colitis, which is worthy of further exploitation.

关 键 词：地塞米松磷酸钠 脂质体 兔 结肠粘膜 体外 Ussingchamber渗透 抗炎药 

分 类 号：R944[医药卫生—药剂学]