rhIL-10对肿瘤坏死因子-α诱导的血管外膜成纤维细胞增殖的影响  

Effects of recombinant human interleukin-10on tumor necorsis factor-α-induced adventitial fibroblast proliferation in vitro

作　　者：欧阳平[1] 孟素荣[1] 刘彦波[1] 许顶立[1] 赖文岩[1] 彭立胜[2] 徐安龙[2] 

机构地区：[1]广州第一军医大学附属南方医院心血管内科,广东广州510515 [2]广州中山大学生命科学学院生物化学系,广东广州510275

出　　处：《第一军医大学学报》2004年第1期50-52,共3页Journal of First Military Medical University

基　　金：广州市科委科技攻关项目(JB00000448165)~~

摘　　要：目的观察重组人白细胞介素-10(rhIL-10)对肿瘤坏死因子-α(TNF-α)诱导的成纤维细胞增殖的影响。方法体外培养NIH/3T3细胞,应用rhIL-10与TNF-α共同作用并设立对照进行比较,采用MTS/PES法确定NIH/3T3细胞的增殖状态。应用流式细胞仪测定细胞周期。结果TNF-α对NIH/3T3细胞增殖具有明显的刺激作用。rhIL-10单独应用对NIH/3T3细胞生长没有影响。在TNF-α刺激下,一定剂量的rhIL-10可抑制NIH/3T3细胞的增殖。rhIL-10可使TNF-α作用下的NIH/3T3细胞大部分处于G0/G1期。结论rhIL-10能明显抑制由TNF-α刺激的成纤维细胞增殖。Objective To observe the effects of recombin an t human interleukin-10 (rhIL-10) on adventitial fibroblast prolifer-ation induce d by tumor necrosis factor-α(TNF-α)in vitro. Methods In this controlled stud y, NIH/3T3 cells cultured in vitro were treated with TNF-αand recombinant human interleukin-10 (rhIL-10), respectively, and the cell proliferation was deter-mi ned by non-radioactive MTS/PES assay. Cell cycle analysis was performed using fl ow cytomertry. Results Compared with the control cells, TNF-αsignificantly sti mulated NIH/3T3 cell proliferation, wherea. rhIL-10 had no such effect. With TNF -αstimulation, rhIL-10 at the dose as low as 1 ng/ml inhibited the proliferatio n of NIH/3T3 cells (P<0.05). The number of cells in G 0 /G 1 phase treated with both TNF-αand rhIL-10 was higher than those treated wit h TNF-αalone (P<0.05), as shown by flow cytometry. Conclusion rhIL-10 can sign ificantly inhibit the proliferation of adventitial fibroblasts induced by TNF-α in vitro.

关 键 词：RHIL-10 肿瘤坏死因子-Α 血管外膜 成纤维细胞 重组人白细胞介素-10 细胞增殖 

分 类 号：R543[医药卫生—心血管疾病]