转sck/cry1Ac双基因抗虫水稻对二化螟和稻纵卷叶螟的抗虫效果  

Resistance of Transgenic Rice Containing Both sck and cry1Ac Genes Against Chilo suppressalis and Cnaphalocrocis medinalis

作　　者：李冬虎[1] 傅强[1] 王锋[2] 姚青[1] 赖凤香[1] 吴进才[3] 张志涛[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006 [2]福建省农业科学院,福建福州350003 [3]扬州大学植物保护系,江苏扬州225009

出　　处：《中国水稻科学》2004年第1期43-47,共5页Chinese Journal of Rice Science

基　　金：国家 8 63计划资助项目 ( 2 0 0 1AA2 12 2 61);国家转基因作物产业化专项资助项目 (J0 0 C 0 0 2 )

摘　　要：通过室内测定和田间观察 ,就转sck/cry1Ac双基因抗虫水稻MSB和MSA对我国主要水稻害虫二化螟和稻纵卷叶螟的抗虫效果进行了研究。室内离体植株测定结果表明 ,MSB、MSA高抗两种害虫 ,抗性水平受水稻苗龄影响。 6 5～71d苗龄水稻上害虫校正死亡率达 98.9%～ 10 0 % ,10 5～ 10 9d时抗性水平有所降低 ,但除MSB对二化螟的校正死亡率 (仍达 79.3% )降低明显 (P <0 .0 5 )外 ,MSA对二化螟及MSB、MSA对稻纵卷叶螟的校正死亡率 (92 .8%～ 98.1% )均无明显下降(P>0 .0 5 )。选取MSB进行的田间实验进一步明确 ,MSB对两种害虫有显著控虫效果 ,抗性水平与抗虫对照克螟稻 1号(含cry1Ab)无明显差异(P >0 .0 5 ) ,且略优于施药亲本明恢 86。相对于未施药明恢 86 ,MSB对两种害虫的防治效果分别为 92 .6 %、97.3% ,施药明恢 86则分别为 87.7%、89.8%。The resistance of two transgenic rices,MSB and MSA containing sck and cry1Ac genes against Chilo suppressalis and Cnaphalocrocis medinalis, two destructive lepidopteran pests of rice in China, was evaluated under both laboratory and field conditions. The results in vitro laboratory bioassay showed that MSB and MSA exhibited high resistance against the two pests, which was influenced by the age of rice plants. The corrected mortality of the two pests were 98.9%-100% on 65-71 days old transgenic rices, and declined in some extent on 105-109 days old ones. However, except the corrected mortality of C. suppressalis on MSB (as high as 79.3%) decreased markedly (P<0.05), those of C . suppressalis on MSA and C . medinalis on both MSB and MSA (92.8%-98.1%) did not decrease significantly. MSB tested in field also showed high resistance to the two pests. There was no significant difference between MSB and the resistant control “Kemingdao 1” (containing a cry1Ab gene). MSB showed a little better controlled effect on the two pests in comparison with its parent control Minghui 86 with insecticides treatment. Compared with Minghui 86 without insecticides treatment, control effects of MSB on the two pests were 92.6% and 97.3%, respectively, while those of insecticides treated Minghui 86 were 87.7% and 89.8%.

关 键 词：转基因 水稻 二化螟 稻纵卷叶螟 抗虫性 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]