疏肝理气活血法对肝郁气滞大鼠阴茎海绵体PDE5活性影响的实验研究  

Experimental Research of PDE5 Activity in Corpora Cavernosa of Ganyu Qizhi Rat Model

作　　者：常德贵[1] 王峥屹[2] 祝彼得[3] 王久源[4] 张蜀武[1] 胡志萍[5] 

机构地区：[1]成都中医药大学附属医院男科,四川成都610072 [2]成都中医药大学中药学博士后流动站,四川成都610072 [3]成都中医药大学病理学教研室,四川成都610072 [4]成都中医药大学男科学教研室,四川成都610072 [5]成都中医药大学

出　　处：《中华男科学杂志》2004年第1期71-73,共3页National Journal of Andrology

摘　　要：目的 :探求肝郁气滞阴茎海绵体组织中Ⅴ型磷酸二酯酶 (PDE5 )活性及疏肝理气活血中药对其影响。　方法 :采用与人类精神性应激十分相似的非损伤性应激刺激法 ,制造肝郁气滞型SD大鼠动物模型 4 0只 ,随机法将大鼠分为 :空白组 ;肝郁证造模组 (简称模型组 ) ;萎康凝胶高剂量给药组 (高剂量组 )和低剂量给药组 (低剂量组 ) 4组 ,每组 10只。以免疫组化与计算机图像分析技术测定海绵体组织中PDE5活性。　结果 :高剂量组阴茎海绵体组织中PDE5活性与模型组差异有显著性 (P <0 .0 1)。　结论 :肝郁气滞可导致阴茎海绵体组织中PDE5活性增强 。Objectives: To study the PDE5 activity in corpora cavernosa of the Ganyu Qizhi model penis and the efffect of the Chinese herbal medicine Shugan Liqi Huoxue (SLH) ointment on it. Methods: Non-injury stress stimulus method similar to human spirit stress was used to extablish the Ganyu Qizhi animal(rat) model, and the PDE5 activity in corpora cavernosa of the rat penis was measured by the method of immunohistochemistry and computer image analysis. Results: The PDE5 activity in corpora cavernosa of the high-dosage SLH group was significantly different from that of the model group (P< 0.01). Conculsion: Ganyu Qizhi may increase the PDE5 activity in corpora cavernosa of the penis while SLH can reduce such activity.

关 键 词：肝郁气滞 阴茎海绵体 萎康凝胶 V型磷酸二酯酶 动物模型 大鼠 男性勃起功能障碍 

分 类 号：R277.52[医药卫生—中医学]