慢性乙肝患者血清细胞因子与肝纤维化的关系  

The Relationship Between Serum Cytokine and Liver Fibrosis in the Patients with Chronic Hepatitis B

作　　者：孙永年[1] 黄祝青[1] 徐斌[1] 黄长形[2] 

机构地区：[1]武警上海总队医院传染科,上海201103 [2]第四军医大学唐都医院感染科

出　　处：《中国医师杂志》2004年第1期41-43,共3页Journal of Chinese Physician

摘　　要：目的　探讨慢性乙肝患者血清细胞因子 (TGF -β1、TNF -α、IFN -γ)在肝纤维化中的作用机制。 方法　采用ELISA和RIA方法检测 95例慢性乙肝患者血清TGF -β1、TNF -α、IFN -γ、HA、PCⅢ、C -Ⅳ的水平 ,并与肝组织病理学变化进行对照研究。 结果　慢性乙肝患者血清TGF -β1、TNF -α、HA、PCⅢ、C -Ⅳ的含量均不同程度高于对照 ,且随肝损害程度的加重而升高 ,与肝损害程度呈正相关 ;并且其水平与肝纤维化程度具有明显的相关性 (P <0 0 5或P <0 0 1)。血清TGF -β1水平与血清HA、PCⅢ、C -Ⅳ水平具有直线相关性 ,而IFN -γ与血清HA、PCⅢ、C -Ⅳ水平呈负相关。结论　慢性乙肝患者血清TGF -β1、TNF -α、IFN -γ水平与肝纤维化相关 。Objective To explore the relationship between serum cytokines and liver fibrosis in patients with chronic hepatitis B(CHB), and to discuss their role in the pathogenesis of liver fibrosis. Methods ELISA and RIA techniques were used to detect the levels of transforming growth factor-β1( TGF-β1),tumor necrosis factor-α(TNF-α), Interferon -γ(IFN-γ), hyaline acid (HA), type III procollagen (PC Ⅲ) and collagen type Ⅳ(C-Ⅳ) in 95 patients with CHB, and contrast study were performed between those serum markers and hepatic pathology. Results The levels of serum TGF-β1, TNF-α, HA, PCⅢ and C-Ⅳ in the patients with CHB were significantly higher than those in normal control group (NC), and were correlated with degree of liver damage positively. Serum levels of TGF-β1, TNF-α, HA, PCⅢ and C-Ⅳ were positive proportion to degree of liver fibrosis (P<0.05 or P<0.01). Serum TGF-β1 level correlated with serum levels of HA, PCⅢ and C-Ⅳ positively. The serum IFN-γ level was negative correlation with HA, PCⅢ and C-Ⅳ. Conclusions The serum levels of TGF-β1, TNF-α and IFN-γ in the patients with CHB were closely correlated with liver fibrosis. Serum TGF-β1, TNF-α and IFN-γ levels are helpful to diagnose liver fibrosis.

关 键 词：慢性乙型肝炎 血清 细胞因子 肝纤维化 作用机制 ELISA RIA 组织病理学 细胞外基质 

分 类 号：R512.62[医药卫生—内科学]