通脉汤对损伤的血管内皮细胞NO生成及纤溶平衡的影响  

The Effects of Tongmai Tang on the Production of NO and the Balance of Fibrinolysis System in Injured Vascular Endothelial Cells

作　　者：何飞[1] 孙明[1] 周宏研[1] 龙开超[1] 李彤[1] 肖长江[1] 

机构地区：[1]中南大学湘雅医院心内科,湖南长沙410008

出　　处：《中国医师杂志》2004年第1期66-67,69,共3页Journal of Chinese Physician

基　　金：湖南省自然科学基金资助项目 (0 2jjy30 34)

摘　　要：目的　研究通脉汤对氧化低密度脂蛋白 (oxLDL)损伤的人脐静脉内皮细胞产生一氧化氮 (NO)及纤溶平衡的影响。方法　制备通脉汤含药血清及oxLDL ,将人脐静脉内皮细胞ECV -3 0 4分为 4组 :⑴空白对照组 ;⑵胎牛血清对照组 ;⑶oxLDL损伤组 :oxLDL终浓度 10 0mg/L ;⑷通脉汤组 :含 5 %通脉汤血清及 10 0mg/LoxLDL ,孵育 2 4h后 ,倒置显微镜观察内皮细胞形态变化 ,测定上清液中NO、纤溶酶原激活物 (t -PA)及纤溶酶原激活物抑制物 -1(PAI -1)浓度。结果　⑴oxLDL组细胞发生损伤改变 ,通脉汤组细胞形态明显好转 ;⑵oxLDL组NO及t -PA浓度低于空白对照组 (P <0 0 5 ) ,通脉汤组NO及t -PA浓度高于oxLDL损伤组 (P <0 0 5 ) ;⑶oxLDL组PAI -1浓度高于空白对照组 ( P <0 0 5 ) ,通脉汤组PAI -1浓度低于oxLDL组 ( P <0 0 5 )。结论　通脉汤含药血清可保护oxLDL损伤的人脐静脉内皮细胞 ,NO生成增加 ,恢复纤溶系统的平衡。Objective To investigate the effects of Tongmai Tang on the production of nitric oxide(NO) and the balance of fibrinolysis system in human umbilical vein endothelial cells(HUVEC) injured by oxidized low density lipoprotein(oxLDL). Methods The herbage-contained serum of Tongmai Tang and oxLDL were prepared, then the human HUVEC-304 cells were divided into 4 groups: ⑴control group; ⑵bovine serum control group; ⑶oxLDL group: 100mg/L oxLDL, and ⑷Tongmai Tang group: 5% herbage-contained serum of Tongmai Tang and 100mg/L oxLDL. After 24h incubation, the morphology of the endothelial cells was observed under the inversion microscope, while the concentrations of NO, t-PA and PAI-1 in the culture medium were measured. Results ⑴The endothelial cells in oxLDL group were seriously injured, while those in Tongmai Tang group got better; ⑵the concentrations of NO and t-PA in the oxLDL group were significantly lower than those in the control group(P<0.05), while the concentrations of NO and t-PA in the Tongmai Tang group were significnatly higher than those in the oxLDL group(P<0.05); ⑶the concentration of PAI-1 in the oxLDL group was significantly higher than that in the control group(P<0.05), while the concentration of PAI-1 in the Tongmai Tang group was significantly lower than that in the oxLDL group(P<0.05). Conclusions Tongmai Tang can protect the endothelial cells from injury induced by oxLDL, and increase the production of NO and restore the balance of the fibrinolysis system.

关 键 词：通脉汤 血管内皮细胞 NO 纤溶平衡 氧化低密度脂蛋白 动脉粥样硬化 冠心病 中医药疗法 

分 类 号：R285[医药卫生—中药学]