四逆汤对高胆固醇喂饲所致动脉粥样硬化形成和氧化损伤的影响  

The Effects of Sini Decoction on Atherosclerosis and Oxidative Injury in Rabbits Fed with Cholesterol Rich Diet

作　　者：吴伟康[1] 黑子清[2] 孙惠兰[1] 梅开勇[3] 罗汉川[1] 

机构地区：[1]中山大学中山医学院中西医结合研究所,广东省广州市510089 [2]中山大学附属第一医院麻醉科 [3]中山大学中山医学院病理学教研室

出　　处：《中国动脉硬化杂志》2003年第6期505-508,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 3 0 0 70 93 0 )资助

摘　　要：为了观察四逆汤对高胆固醇喂饲所致家兔实验性动脉粥样硬化形成和氧化损伤的影响 ,采用高胆固醇喂饲模型 ;将 2 4只新西兰家兔随机分为 3组 :正常组、模型组、四逆汤组 ,分别给予普通饲料 (正常对照组 )和高胆固醇饮食 (其它 2组 )喂养 ,四逆汤组另加用四逆汤方药 ;实验结束后 ,观察血管病理变化、血和血管超氧化物歧化酶活性、血浆和血管丙二醛含量、超氧化物歧化酶蛋白和基因表达。结果发现 ,与模型组相比 ,四逆汤组主动脉脂质斑块面积、内膜斑块面积和厚度明显降低 ;血和血管超氧化物歧化酶活性明显增加 ;血浆和血管丙二醛的含量明显增加 ;血管超氧化物歧化酶蛋白和基因表达明显增强。研究结果提示 ,四逆汤具有较好的抗动脉粥样硬化作用 ,抗氧化损伤可能是四逆汤产生抗动脉粥样硬化作用的重要机制之一。Aim To observe the effects of Sini decoction on atherosclerosis and oxidative injury in rabbits fed with cholesterol rich diet. Methods 24 rabbits were randomly divided into three groups. Normal group was fed with normal diet; Model group was fed 1% cholesterol and 5% fat diet; Sini decoction group was fed 1% cholesterol and 5% fat diet plus Sini decoction [2.1 g/(kg·d)]. At the end of study, the plaque area were measured, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of blood and aorta were detected. SOD protein and gene expression were observed with immunohistochemistry and hybridization in situ method. Results Compared with model group, Sini decoction could diminish lipid plaque area, intimal plaque area and intimal thickness, reduce the levels of aorta and serum MDA, increase aorta and serum SOD activity, increase the expressions of SOD protein and SOD gene in aorta. Conclusions The experimental results suggested that Sini decoction had antiatherogenic effects. The possible mechanism might be that Sini decoction could reduce oxidative injury in rabbits.

关 键 词：药理学 四逆汤 动脉粥样硬化 病理学检查 超氧化物歧化酶 丙二醛 主动脉 动物模型 胆固醇 

分 类 号：R285.5[医药卫生—中药学] R259.435[医药卫生—中医学]