转染IL-3基因的骨髓基质细胞促进骨髓移植小鼠造血功能重建  

The Improving Effect of Bone Marrow Stromal Cell Transfected with IL-3 gene on Hematopoietic Reconstitution in Bone Marrow Transplantation of Mice

作　　者：蒋激扬[1] 李爱玲[1] 王光明[1] 马建波[1] 郝洁[1] 关志强[1] 谢蜀生[1] 

机构地区：[1]北京大学医学部免疫系,北京100083

出　　处：《中国实验血液学杂志》2003年第6期633-638,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目 编号 3 0 170 895

摘　　要：为了探讨可调控表达IL 3基因的骨髓基质细胞系对异基因骨髓移植小鼠造血功能重建的促进作用 ,建立了可诱导表达IL 3基因的工程化骨髓基质细胞系QXMSC1tet on +IL 3,加入多西环素 (Dox)诱导骨髓基质细胞系表达IL 3并检测其活性。C5 7BL/ 6 (H 2 b)小鼠经γ射线致死剂量照射后 ,输入供体BALB/C(H 2 d)小鼠去除T细胞的骨髓细胞 1× 10 7/只 ,同时输注骨髓基质细胞QXMSC1tet on +IL 35× 10 5/只 ,并灌服多西环素诱导IL 3表达。在第 30天 ,6 0天检测骨髓移植小鼠外周血红细胞 ,白细胞数 ,骨髓有核细胞数 ,骨髓CFU S ,CFU GM ,CFU E和CFU GEMM数。结果表明 :成功建立可诱导表达IL 3基因的骨髓基质细胞系QXMSC1tet on +IL 3,异基因骨髓移植共输注基质细胞系QXMSC1tet on +IL 3可使异基因骨髓移植小鼠外周血红细胞、血小板、白细胞明显恢复 ,骨髓中有核细胞数 ,CFU S ,CFU GM ,CFU E ,CFU GMEE明显增加。结论 :输注基因工程化基质细胞 ,并诱导细胞因子ILTo study the improving effect of regulatable gene of IL 3 engineered bone marrow stromal cell on the hematopoietic reconstitution in allogeneic bone marrow transplantation, an inducible gene expression system was established in a bone marrow stromal cell line which expressed IL 3 gene induced by doxycycline (Dox). The lethally irradiated mice C57BL/6 (H 2 d) were co transplanted with allogeneic bone marrow (BALB/c, H 2 d, 1×10 7/mice) in which T cell were depleted by monoclonal antibody anti Thy1.2 added with complement and the gene engineered stromal cell QXMSC1tet on+IL 3 (5×10 5/mice) at the same time. Dox was administrated continuously for 15 days to induce the expression of IL 3. The hematopoiesis in the bone marrow transplanted mice were observed at 30, 60 days post transplantation, respectively. The numbers of RBC and WBC in peripheral blood were counted, and nucleated cells, CFU S, CFU GM, CFU E and CFU GEMM were measured in recipient bone marrow. The results showed that the engineered stromal cell line achieved high level and controllable IL 3 expression. Co graft with QXMSC1tet on+IL 3 significantly increased the number of RBC, WBC in recipient peripheral blood, and the nucleated cells, CFU S, CFU GM, CFU E, CFU GEMM in bone marrow, compared with those coinfused with QXMSC1 or QXMSC1tet on TRE as control. In conclusion, regulatable gene IL 3 engineered bone marrow stromal cells accerelates hematopoietic reconstitution after allogeneic bone marrow transplantation.

关 键 词：IL-3基因 骨髓基质细胞 骨髓移植 造血功能 治疗 小鼠 细胞数 

分 类 号：R392.4[医药卫生—免疫学] R331.2[医药卫生—基础医学]