石斛总DNA的提取及鉴定  

Extraction and Characterization of Total DNA from Dendrobium

作　　者：彭锐[1] 宋洪元[2] 李泉森[1] 王宇[1] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]西南农业大学园艺系,重庆400716

出　　处：《中国中药杂志》2003年第12期1129-1131,共3页China Journal of Chinese Materia Medica

基　　金：重庆市科委应用基础课题 (2 0 0 0 672 12 )

摘　　要：目的 :探讨不同DNA提取方法对石斛属DNA质量及分子标记结果的影响。方法 :对提取的DNA进行紫外光谱分析、总DNA电泳及RAPD分析。结果 :不同方法提取的DNA质量及产率存在显著差异 ,对RAPD结果影响很大。结论 :石斛属植物在DNA提取前加入无CTAB缓冲液抽提多糖等杂质 ,可获得较高质量的DNA。Objective: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR. Method: the electrophoresis of total DNA ,UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods. Result: The DNA concentration and yields were different,which greatly influenced the result of RAPD-PCR. Conclusion: The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.

关 键 词：总DNA 提取 鉴定 石斛属 紫外光谱 

分 类 号：R284[医药卫生—中药学]