高效液相色谱法测定五味子属药用植物木脂素的含量  

Determination of Lignans of Schisandra Medicinal Plants by HPLC

作　　者：王彦涵[1] 高建平[1] 陈道峰[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海200032

出　　处：《中国中药杂志》2003年第12期1155-1160,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目 (3 0 2 715 86) ;高校博士点基金项目 (2 0 0 0 0 2 65 19) ;高校优秀青年教师教学和科研奖励基金项目 (1999~ 71)

摘　　要：目的 :对五味子属植物的木脂素成分进行含量测定 ,探讨五味子药物资源问题。方法 :用HPLC对五味子属 14个种 (变种 ) 18个样品 (果实、茎藤和根 )中木脂素成分五味子醇甲 (schisandrin) ,五味子醇乙 (gomisinA) ,五味子酯甲 (schisantherinA) ,五味子甲素 (deoxyschizandrin) ,表 加巴辛 (d epigalbacine) ,安五脂素 [(+) anwulig nan],五味子乙素 (wuweizisuB) ,6 O 苯甲酰戈米辛O(6 O benzoylgomisinO)和五味子丙素 (wuweizisuC)进行含量测定。结果 :5种不同种的五味子果实中 ,9种木脂素总量为 0 .5 2 %～ 1.96 % ,其中以五味子和华中五味子含量最高 ,11种 (包括变种 )茎的 9种木脂素总量为 0 .0 2 %～ 1.5 1% ;小花五味子果实含有与华中五味子相似的木脂素成分 ,其中甲素的含量较高 (0 .5 9% ) ;绿叶五味子与五味子成分相似 ,其醇甲、醇乙和乙素的含量均较高 ;翼梗五味子果实、华中五味子果实、小花五味子茎、毛脉五味子茎和二色五味子根的安五脂素含量较高 ,分别 0 .77% ,0 .4 2 % ,0 .5 0 % ,0 .2 6 % ,0 .38% ;毛脉五味子茎、毛叶五味子茎和金山五味子茎的表 加巴辛含量较高 (分别为0 .89% ,1.5 1% ,0 .17% )。结论 :五味子属植物果实的木脂素含量高于根和茎 ;翼梗五味子果实、小花五味子茎?Objective: To determinate the contents of lignans in the stems, roots and fruits of Schisandra medicinal plants. Method: HPLC was applied to determine the contents of schisandrin, gomisin A, schisantherin A, deoxyschizandrin, d-epigalbacine, (+)-anwulignan, wuweizisu B, 6- O -benzoylgomisin O and wuweizisu C in the stems, roots and fruits of Schisandra . Result: The percent contents of 9 lignans in the fruits of 5 species were 0.52%～1.96%, and 0.02%～1.51% in the stems of 11 species. The lignans in the fruits of S. micrantha were similar to that of S. sphenanthera , especially with high content of deoxyschizandrin. The lignans of S. viridis were similar to that of S. chinensis and with high content of schisandrin, gomisin A and wuweizisu B. (+)-Anwulignan was present in the fruits of S. henryi and S. sphenanthera , in the stems of S. micrantha and S. pubescens var. pubinervis , and in the roots of S. bicolor with percentages of 0.77%, 0.42%, 0.50%, 0.26% and 0.38% respectively. d-Epigalbacine was present in the stems of S. pubescens var. pubinervis , S. pubescens and S. glaucescens with percentages of 0.89% , 1.51% and 0.17% respectively. Conclusion: The lignans contents in the fruits of Schisandra were higher than that in the roots and stems. The fruits of S. henryi and S. sphenanthera , the stems of S. micrantha and S. pubescens var. pubinervis and the roots of S. bicolor , the stems of S. pubescens var. pubinervis , S. pubescens and S. glaucescens may be served as the resources plants of (+)-anwulignan and d-epigalbacine respectively.

关 键 词：高效液相色谱法 含量测定 五味子属 药用植物 木脂素 

分 类 号：R284.1[医药卫生—中药学]