聚合酶链反应-单链构型多态性检测细胞壁缺陷型淋病奈瑟菌cppB基因变异  

DETECTION THE CRYPTIC PLASMID B GENE VARIATION IN NEISSERIA GONORRH OEAE L-FORMS BY PCR-SINGLE STRAND CONFORMATION POLYMORPHISM

作　　者：魏荣旋[1] 王和[2] 

机构地区：[1]贵州遵义医院中心实验室,遵义563002 [2]贵阳医学院微生物学教研室,贵州贵阳550004

出　　处：《中国微生态学杂志》2003年第6期329-330,共2页Chinese Journal of Microecology

摘　　要：目的 :检测与分析淋病奈瑟菌 L 型的 cpp B基因 ,探讨细胞壁缺陷对淋病奈瑟菌 cpp B基因的影响。方法 :用青霉素诱导淋病奈瑟菌成为 L 型并获得稳定 L 型纯培养物 ,用 cpp B基因特异性引物以聚合酶链反应 (PCR)检测稳定 L型纯培养物的 cpp B基因和进行单链构型多态性 (SSCP)分析。结果 :淋病奈瑟菌的细菌型及其 L型都具有 cpp B基因扩增产物 ,但 PCR-SSCP分析可见异常泳动 DNA带型 (细菌型有 2条带、L型有 3条带 )。结论 :细胞壁缺陷淋病奈瑟菌仍然具有 cpp B基因 。Objective:To detect the cppB gene in L - forms of Neisseria gonorrhoeae and probe the influence of cell wall deficien cy on the Cryptic Plasmid B gene of N gonorrhoeae Methods:The N gonorrhoeae was induced into L-forms by p enicillin and the cppB gene of the pure culture of L-form was determined by PCR and SSCP.Results:By PCR,the cppB genes would be found in both the bacter ial form and the L-forms of N gonorrhoeae There were two bands in the ba cterial form and three bands in L-forms by PCR-SSCP.Conclusion:The cppB gene is still contained in the cell wall de ficient N gonorrhoeae but its base alignment would be changed.

关 键 词：SSCP 淋病奈瑟菌L型 cppB基因 聚合酶链反应-单链构型多态性检测 细胞壁缺陷型 

分 类 号：R378.16[医药卫生—病原生物学] R759.2[医药卫生—基础医学]