棘胸蛙消化道内分泌细胞的免疫组织化学定位  

Immunohistochemistry of endocrine cells in the digestive tract of Paa spinosa

作　　者：张盛周[1] 黄徐根[1] 吴孝兵[1] 

机构地区：[1]安徽师范大学生命科学学院

出　　处：《动物学报》2003年第6期858-864,共7页ACTA ZOOLOGICA SINICA

基　　金：安徽教育厅科学基金 (No .2 0 0 1kj0 99);重要生物资源保护与利用安徽省重点实验室专项基金;安徽省自然科学基金资助(0 10 3 45 0 1);安徽省优秀青年基金资助~~

摘　　要：We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin peroxidase method) using eight gut hormone antisera. The 5 hydroxytryptamine immunoreactive cells were distributed throughout the digestive tract with the highest density in the stomachus pyloricus, the second highest in the duodenum, fewer in the oesophagus, stomachus cardiacus and rectum. The gastrin immunoreactive cells were located mainly in the stomachus pyloricus and occasionally in different parts of the intestine. The somatostatin immunoreactive cells occured mainly in the stomach, frequently in the stomachus pyloricus, and occasionally in different parts of the intestine. The pancreatic polypeptide immunoreaetive cells were found with the highest density in the duodenum, the second highest in the stomachus cardiacus, and rarely in the rectum. No immunoreactive cells were observed with the antisera to glucagon, substance P, growth hormone and calcitonin. but there were glucagon and substance P mucosal nerve plexus throughout the digestive tract, and both with the highest density in the duodenum .We studied the distribution and density of the endocrine cells in the digestive tract of Paa spinosa using immunohistochemical method (streptavidin peroxidase method) using eight gut hormone antisera. The 5 hydroxytryptamine immunoreactive cells were distributed throughout the digestive tract with the highest density in the stomachus pyloricus, the second highest in the duodenum, fewer in the oesophagus, stomachus cardiacus and rectum. The gastrin immunoreactive cells were located mainly in the stomachus pyloricus and occasionally in different parts of the intestine. The somatostatin immunoreactive cells occured mainly in the stomach, frequently in the stomachus pyloricus, and occasionally in different parts of the intestine. The pancreatic polypeptide immunoreaetive cells were found with the highest density in the duodenum, the second highest in the stomachus cardiacus, and rarely in the rectum. No immunoreactive cells were observed with the antisera to glucagon, substance P, growth hormone and calcitonin. but there were glucagon and substance P mucosal nerve plexus throughout the digestive tract, and both with the highest density in the duodenum .

关 键 词：棘胸蛙 消化道 内分泌细胞 免疫组织化学定位 

分 类 号：S917[农业科学—水产科学]