鸡垂体前叶提取物诱导鸡超数排卵  

Chicken anterior pituitary extract induces multiple ovulate in the domestic fowl (Gallus domesticus)

作　　者：韩海棠[1] 赵晨[1] 李哲[1] 沙金[1] 周振明[1] 张易祥[1] 钱长嵩[1] 李赞东[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《动物学报》2003年第6期865-867,共3页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金 (No .3 0 14 0 0 0 2 );国家高技术研究发展计划 (No.2 0 0 1AA2 13 13 1)资助项目~~

摘　　要：Chicken anterior pituitary extract(CAPE) and acetone dried chicken anterior pituitary (ACAPE) were injected intraperitoneally into normal laying hens (‘ovulation suppressed’ following pretreatment with daily subcutaneous injection of PMSG) to induce multiple ovulations. The dose of PMSG, the effect of CAPE and ACAPE and the time required for induction of ovulation following injection of ovulation inducing hormone were determined. The results revealed that (1) when 75 IU PMSG was administered daily, egg laying stopped in 33% of the treated hens within 6 days after the first injection. However, the percentage of hens showing the same effects changed significantly (over 95%) within 3 to 6 days when the amount of PMSG was increased to 100 IU; (2) the number of ovulated ova was 1 00±0 00, 2 33±0 26,2 20±0 20 respectively after receiving 100 mg, 200 mg and 300 mg; the number of ovulated ova was 2 00±0 00, 2 86±0 48, 3 00±1 50 respectively after receiving 10 mg, 15 mg and 20 mg ACAPE; (3) The time from injection to ovulation in almost all hens was about 7 5 h except one hen ovulated about 6 5 h after receiving ACAPE .Chicken anterior pituitary extract(CAPE) and acetone dried chicken anterior pituitary (ACAPE) were injected intraperitoneally into normal laying hens (‘ovulation suppressed’ following pretreatment with daily subcutaneous injection of PMSG) to induce multiple ovulations. The dose of PMSG, the effect of CAPE and ACAPE and the time required for induction of ovulation following injection of ovulation inducing hormone were determined. The results revealed that (1) when 75 IU PMSG was administered daily, egg laying stopped in 33% of the treated hens within 6 days after the first injection. However, the percentage of hens showing the same effects changed significantly (over 95%) within 3 to 6 days when the amount of PMSG was increased to 100 IU; (2) the number of ovulated ova was 1 00±0 00, 2 33±0 26,2 20±0 20 respectively after receiving 100 mg, 200 mg and 300 mg; the number of ovulated ova was 2 00±0 00, 2 86±0 48, 3 00±1 50 respectively after receiving 10 mg, 15 mg and 20 mg ACAPE; (3) The time from injection to ovulation in almost all hens was about 7 5 h except one hen ovulated about 6 5 h after receiving ACAPE .

关 键 词：鸡 鸡垂体前叶提取物 孕马血清 超数排卵 

分 类 号：S831.3[农业科学—畜牧学]