武汉地区不同人群血清HCV感染状况研究  

Prevalence of HCV Infection in Different Populations of Wuhan Area

作　　者：郝连杰[1] 郭林生[1] 张永东 Theilmann L 杨泽川[4] Takada A 

机构地区：[1]同济医科大学附属同济医院临床免疫研究室 [2]武汉市传染病医院病理室 [3]德国海德堡大学内科学院消化内科 [4]同济医科大学附属协和医院传染病教研 [5]本金沢医科大学消化器内科

出　　处：《同济医科大学学报》1992年第3期145-149,共5页Acta Universitatis Medicinae Tongji

摘　　要：采用Ortho抗HCV ELISA C100-3试剂对武汉地区不同人群进行了抗HCV筛选。部分阳性标本作了RIBA证实实验及HCV RNA分析。并分析了5例HCV或HBV/HCV感染者的HCV基因型。结果表明:①在献血人群中,经RIBA证实抗HCV检出率为1.20％,HCV病毒血症者为0.80％;②多次受血治疗组抗HCV检出率为13.64％;③肝病组,重症肝炎为32.00％,HCC为22.86％,CPH和轻型CAH为4.05％,婴儿肝炎综合征中为5.00％;④亚基因探针杂交结果显示:武汉地区肝病患者感染的HCV主要为HCV-K2型(60.00％)。表明:经HBsAg和ALT筛选的合格献血员血清中HCV感染率仍较高,除接受血治疗外,重症肝炎和HCC患者对HCV易感性也较高,在婴儿肝炎综合征中约5％为HCV感染引起;初步结果提示武汉地区HCV的基因型主要为HCV-K2型。Anti-HCV C100-3 was dectected by the Ortho ELISA diagnostic system in differenent populations of Wuhan area. The positive samples were submitted to RIBA confirmation and RT-HCV RNA PCR assay. HCV RNA gerotyp-ing in 5 patients with HCV or HCV/HBV infection was also determined by sub-gene-probe slot blot. It was found that: 1. prevalence of anti-HCV was 1.20 % and that of virusemia was 0.80 % as confirmed by RIBA; 2. the detective rate was 13.64 % in the group with multiple recipients; 3. prevalence of HCV infection was 32.00 % in patients with severe hepatitis, 22.86 % in those with HCC, 4.05 % in those with mild chronic hepatitis B, and 5 in those with syndrome of infant hepatitis; and 4. the major genotyping of HCV in Wuhan area was found to be HCV-K2 by our primary study. These findings indicate that the blood donors screened by HBsAg and ALT still had high HCV infection. The patients with severe hepatitis and HCC exhibited high susceptibility, and HCV infection may account for 5.00% among the causative agents of syndrome of infant hepatitis. From the above it follows that the major genotypirg of HCV occurring in Wuhan area is HCV-K2.

关 键 词：聚合酶链反应 基因型 丙肝病毒 

分 类 号：R512.630.2[医药卫生—内科学]