蓖麻蚕卵黄磷蛋白鉴定、纯化及抗血清制备  

IDENTIFICATION AND PURIFICATION OF THE VITELLIN AND PREPARATION OF THE ANTISERUM IN CASTOR SILKWORM PHILOSAMIA CYNTHIA RICINA

作　　者：赵小凡[1] 王金星[1] 李根君[1] 

机构地区：[1]山东大学生物系

出　　处：《华东昆虫学报》1995年第1期81-84,共4页Entomological Journal of East China

基　　金：山东大学青年科学基金;国家教委留学回国人员基金

摘　　要：本文通过聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）法，分析了蓖麻蚕卵黄蛋白质组分，对卵黄磷蛋白（ＶｉｔｅｌｌｉｎＶｎ，）进行了鉴定、纯化，并制备了抗血清。对卵黄磷蛋白的分子量及相对含量进行了测定。并证明卵黄磷蛋白若处在酸性ｐＨ条件下能降解。The coniposition of the yolk proteins was analyLed by the method of SDS PAGE. The vitellin was identified and purified,and the antiserum to the vitellin wasprepared;Ttie niolecular weight of the vItellin was determined。Meanwhile,the relativequantity of the vitellin was calculated.The vitellin was also found to be hydrolyzed at aciticpH condition。

关 键 词：蓖麻蚕 卵黄磷蛋白 鉴定 纯化 抗血清制备 

分 类 号：S881.24[农业科学—特种经济动物饲养] Q966[农业科学—畜牧兽医]