RNA干扰作用及其技术  

RNA interferenceand its technology

作　　者：王贤[1] 滑冰[2] 

机构地区：[1]周口师范学院生物系,河南周口466000 [2]商丘师范学院生物系,河南商丘476000

出　　处：《周口师范学院学报》2003年第5期47-50,共4页Journal of Zhoukou Normal University

摘　　要：RNA干扰作用(RNAi)是通过双链RNA介导的特异性转录后基因沉默,这一过程已在拟南芥、线虫和真菌等多种模式生物中得到揭示.RNAi主要通过双链RNA被核酸酶切割成的小干扰RNA(siRNA),由siRNA介导识别并靶向切割同源靶mRNA分子而实现的.RNAi具有高效性和高特异性,已成为关闭基因的一项新技术,在基因功能研究和疾病的基因治疗中具有广阔的应用前景.RNA interference(RNAi) is something of post-transcriptional gene silencing, specifically mediated by double-stranded RNA. RNAi was observed in many model organisms, such as Arabidopsis, C. elegansand fungi. The basic process in RNAi involves a dsRNA that is processed by endonuclease into small interference RNA (siRNA) that guides recognition and targeted cleavage of homologous target mRNA. Being effective and highly specific ,RNAi has becames a novel technique in knocking gene down plays important roles in gene function study and gene therapy diseases.

关 键 词：基因 RNA干扰 DICER酶 基因沉默 关闭基因 

分 类 号：Q78[生物学—分子生物学]