植物原生质体培养和体细胞融合技术研究进展  

Advances in the culture of plant protoplast and techniques of somatic hybridization

作　　者：李杰[1] 黄敏仁[1] 王明庥[1] 蔡汝 

机构地区：[1]南京林业大学林木遗传和基因工程国家林业局重点实验室,江苏南京210037 [2]江苏省兰花工程研究中心,江苏泰州225321

出　　处：《仲恺农业技术学院学报》2003年第4期64-71,共8页Journal of Zhongkai Agrotechnical College

基　　金：国家"863"高技术研究计划(2001AA244011)资助项目.

摘　　要：自20世纪70年代以来,植物原生质体培养和体细胞融合操作技术不断趋于精密化、微量化、高效化与多样化.文章侧重于实验操作技术和当前研究热点,对植物原生质体培养和体细胞杂交等关键技术环节的重要研究成果和研究进展进行了综述,并对其在理论和实践上的应用前景及未来发展趋势进行了展望.Since 1970's,the technique of plant protoplast culture and somatic hybridization gradually trend to precision,profoundness,efficiency and diversification. Emphasized particularly on the techniques of experiment operation and the focuses of recent researches, advances in protoplast culture and somatic hybridization of plants were reviewed in this paper, and their prospects in the applications of theories and practice were also introduced.

关 键 词：植物 原生质体培养 体细胞融合技术 研究进展 发展趋势 植株再生 

分 类 号：Q943.1[生物学—植物学]