SSR标记鉴定栽培稻杂种F_1花粉愈伤组织基因型的偏态分离  

Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F_1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers

作　　者：姚焱[1] 卢永根[1] 刘向东[1] 冯九焕[1] 张桂权[1] 

机构地区：[1]华南农业大学植物分子育种研究中心,广州510642

出　　处：《中国农业科学》2003年第12期1427-1431,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 ( 39970 0 4 8);广东省自然科学基金资助项目 ( 990 70 7);霍英东教育基金资助项目 (71 0 2 1 )

摘　　要：分别对栽培稻F1花粉不育基因S a、S b和S c单座位内互作杂种F1进行离体花药培养 ,利用SSR分子标记鉴定了花粉愈伤组织的基因型。结果表明 ,不同座位F1产生的携带花粉育性基因Si 或Sj 的花粉形成愈伤组织的能力偏离 1∶1的分离比 ,与自然条件下F2 群体偏离方向不一致。携带不同花粉不育基因座位的F1产生花粉愈伤组织基因型的偏离方向有差异 ,表现为S a和S c单座位内互作杂种F1的花粉愈伤组织偏向Sj,Si∶Sj 比例分别为 1∶4.81和 1∶1.96;S b单座位内互作杂种F1花粉愈伤组织偏向Si,Si∶Sj 比例为 1∶0 .3 5。研究培养条件对偏态分离的影响 ,发现预冷处理可明显提高偏态分离的程度 。S-a, S-b and S-c are three loci for F 1 p ol len sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) are al l S j/S j at these three loci, while its F 1 pollen sterile near-isogeni c lines, TISL2(S-b), TISL4(S-a) and TISL5(S-c) are S i/S i according to their respective sterility locus. Using SSR molecular marker to det ect the segregation of the allele S i and S j in pollen calli populati on induced from different hybrid F 1 which having different pollen sterility lo cus, it showed that the segregation of allele S i and S j was distorte d. The distorted direction of pollen calli population in vitro was not as th e same as F 2 population in vivo. The quantities of pollen callus carrying S j were much more than that of carrying S i at S-a and S-c locus, the ratio of S i and S j were 1∶4.81 and 1∶1.96. But the opp osite tendency was showed at S-b locus, the ratio of S i and S j being 1∶0.35. At the same time, all these results were undisturbed by either cu lture medium or culture period.

关 键 词：SSR标记 鉴定方法 栽培技术 稻 杂种F1代 花粉愈伤组织 基因型 偏态分离 

分 类 号：S511.2[农业科学—作物学]