RGD抗体的研制和其在丝素膜中的应用  

ANTIBODIES AGAINST RGD INDUCED IN RABBIT AND ITS APPLICATION IN SILK MATERIALS

作　　者：李茂林[1] 杨新林[1] 朱鹤孙[1] 王松[1] 范翠红[1] 赵军[2] 孙立忠[2] 

机构地区：[1]北京理工大学,北京100081 [2]中国协和医科大学阜外心血管病医院,北京100037

出　　处：《中国生物医学工程学报》2003年第6期537-542,共6页Chinese Journal of Biomedical Engineering

基　　金：国家重点基础研究发展规划资助项目 (G19990 64 7)

摘　　要：本研究应用双功能偶联剂小分子间苯二甲基二异氰酸盐 (m xylylen diisocyanate ,简称XDI)作桥梁 ,将短肽RGD(GLY ARG GLY ASP SER PRO LYS)粘附生长因子偶联到卵清白蛋白 (Ovalbumin ,简称OVA)载体上 ,对偶联物RGD OVA经SDS 聚丙烯酰胺凝胶纯化和紫外光谱测定 ,确定RGD与OVA偶联比为 11∶1,用偶联物对兔子进行背部小剂量多点免疫注射 ,诱发出抗RGD抗体 ,利用得到的兔抗RGD抗体IgG包埋于丝素材料内 ,并借助抗原抗体的结合力 ,结合RGD在该丝素膜表面 ,对这种结合有RGD抗体的丝素膜进行培养血管内皮细胞(Endothelialcell,简称EC)的试验 ,结果表明 ,结合有RGD的丝素膜与其对照组相比 ,细胞数量有显著提高。This study aimed at the biological modifications of silk membranes to create a novel biomaterial in order to improve membrane compatibility and promote its clinical application, especially in cardiovascular grafting. The adhesive and growth protein, Arg-Gly-Asp(RGD) plays an important role in cell adhesion, migration and growth. The study tried to bury the immunoprotein IgG of RGD in silk solution, yielding a fibroin membrane binding RGD through IgG affinity. As RGD is a hapten, the study used the ovalbumin as the carrier and conjugated RGD to the ovalbumin, it was proved that the cptimal binding ratio was 11 to 1. It was dermonstrated that the RGD bound silk membrane stimulated growth of the endothelial cells and promoted tissue regeneration in the silk scaffold.

关 键 词：RGD 抗体 半抗原 丝素膜 血管内皮细胞 

分 类 号：R318[医药卫生—生物医学工程] Q503[医药卫生—基础医学]