生物材料改性对人涎腺细胞生长的影响  

EFFECT OF MODIFIED BIOLOGICAL MATERIALS ON THE GROWTH OF SALIVARY CELL LINE

作　　者：朱劼[1] 杨新林[1] 孙涛[2] 王松灵[2] 

机构地区：[1]北京理工大学生命科学与技术学院 [2]首都医科大学附属北京口腔医院,北京100050

出　　处：《中国生物医学工程学报》2003年第6期543-547,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 3 0 170 2 5 4);北京市科技项目 (H0 10 910 0 10 113 )资助项目

摘　　要：目的 :利用SO2 等离子体对聚乳酸和聚醚酯膜进行表面改性 ,研究人类涎腺细胞系HSG细胞在生物材料上的生长。方法 :材料的表面性质通过表面接触角和X射线光电子能谱 (XPS)进行表征。细胞形态与生长情况则通过倒置显微镜观察和MTT检测来评估。结果 :用SO2 等离子体改性后 ,膜表面接枝上磺酸基团 ,亲水性明显增强 ;在几种材料中 ,用SO2 等离子体改性后的聚醚酯膜更适合HSG细胞的粘附生长。结论 :用SO2 等离子体改性后的聚醚酯材料可能用于人造涎腺的支架材料。Objective: To investigate the growth of human salivary gland (HSG) cells on the modified biomaterials. Methods: Surface modification of polylactic acid (PLA) and poly(ethylene oxide terephtalate)/poly (butylene terephtalate) (PEOT/PBT) membranes were accomplished by SO2 plasma treatment. Surface properties of materials were characterized by contact angle and X-ray photoelectron spectrum (XPS) analyses. Cell morphology and growth were observed under inverted microscope and evaluated by MTT test, respectively. Results: Sulfonic groups were chemically attached to the membrane surfaces after SO 2 plasma treatment, and the hydrophilicity of the membranes was remarkably improved. The modified PEOT/PBT membrane was found to be the most suitable for the adhesion and growth of HSG cells. Conclusion: The PEOT/PBT polymer modified by SO2 plasma may have a potential use as the scaffords for artificial salivary gland.

关 键 词：聚乳酸 聚醚酯 S02等离子体 HSG细胞 细胞生长 生物材料 表面改性 

分 类 号：R318.08[医药卫生—生物医学工程]