褐飞虱对甲胺磷的抗性遗传  

The inheritance of resistance to methamidophos in brown planthopper, Nilaparvata lugens Stal

作　　者：刘泽文[1] 韩召军[1] 张玲春[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,南京210095

出　　处：《植物保护学报》2003年第4期407-412,共6页Journal of Plant Protection

基　　金："973"国家重点基础研究项目(J20000162)

摘　　要：室内抗性筛选表明,褐飞虱对甲胺磷抗性的发展呈不规则的“S”形,第5代前抗性上升很慢,第5～15代间迅速上升,第15代后上升再次变慢。利用选育的抗甲胺磷褐飞虱品系和敏感品系,研究了褐飞虱对甲胺磷的抗性遗传形式。结果显示,正交和反交子代的显性度D值分别为0.5130和0.4673,表明抗性主效基因是不完全显性遗传的。利用剂量对数-死亡机率值曲线分析法,初步判断抗性遗传不是单基因控制的;卡方(X^2)测验进一步证实,抗性遗传可能是两个或两个以上的基因控制。抗性筛选全过程和F_5～F_(13)的现实遗传力(h^2)分别为0.2190和0.2457,说明褐飞虱对甲胺磷抗性产生的速率较快,出现高抗性的风险较大。Methamidophos resistance of brown planthopper (BPH) was selected in laboratory. The results showed that the velocity of the resistance development was approximatively shaped as the letter ' S ' . The resistance changed little before the fifth generation and after the fifteenth generation, and the sharp change occurred between the fifth and fifteenth generations. The mode of inheritance of resistance to methamidophos in BPH was evaluated from laboratory-reared susceptible strain (S) and resistant strain (R) after 17 generations selection. Degrees of dominance (D) are0.5130 ( R× S) and 0.4673 ( S× R), which indicated that the major resistance gene involved is incompletely dominant. Log dosage-probit mortality curves indicated that methamidophos resistance in BPH appeared to be controlled by two or more genes, which was further proved by X2 test. The realized heritability (h2) of F1 - F17 and F5 - F13 were 0.2190 and 0. 2457 respectively, which indicated that the resistance rate and potential of methamidophos resistance in BPH are fast and high.

关 键 词：褐飞虱 甲胺磷 抗性遗传 室内抗性筛选 选育 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]