脑缺血-再灌注损伤时MMP9表达及养阴通脑颗粒干预作用的研究  

Study of matrix metalloproteinase 9 expression after mice ischemia/reperfusion in hippocampus and treatment effects of Yangyin Tongnao granule(养阴通脑颗粒)

作　　者：杜月光 万海同 孟祥磊 张宇燕[2] 余道军 白海波 别晓东[2] 姚建龙 

机构地区：[1]浙江中医学院,浙江杭州310053 [2]浙江大学,浙江杭州310009 [3]浙江康恩贝制药股份有限公司,浙江杭州310023

出　　处：《中国中西医结合急救杂志》2003年第6期330-332,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 941);浙江省自然科学基金青年科技人才培养专项资金项目 ( RC0 0 0 5 0;3 0 14 98)

摘　　要：目的 :观察小鼠脑缺血再灌注后海马区基质金属蛋白酶 (MMPs)表达情况 ,探讨其在脑缺血损伤中的作用以及养阴通脑颗粒对脑缺血损伤保护作用的机制。方法 :应用免疫组织化学技术观察脑缺血再灌注后海马区基质金属蛋白酶 9(MMP9)蛋白表达的变化。结果 :模型组缺血再灌注 2 0 min后 CA1区的 MMP9表达明显增加 ,其阳性细胞数平均为 (4 2 .3± 17.1)个 ,与假手术组 (4 .8± 1.6 )个比较有明显差异 (P<0 .0 5 )。养阴通脑颗粒、尼莫地平可使 MMP9表达明显降低 ,其阳性细胞数分别为 (7.2± 2 .9)个和 (12 .7± 5 .3)个 ,与模型组比较 P均 <0 .0 5。结论 :脑缺血再灌注可诱导 CA1区的 MMP9表达增加 ;MMP9表达增加可能是脑缺血再灌注损伤的机制之一 ;养阴通脑颗粒可抑制 MMP9表达 ,对脑缺血再灌注损伤具有保护作用。Objective: To investigate the expression of matrix metalloproteinase 9(MMP9) after cerebral ischemia/reperfusion in hippocampus, and to explore the role of MMP9 during cerebral ischemia/reperfusion and the treatment effect of Yangyin Tongnao granule(养阴通脑颗粒) on the brain injury induced by cerebral ischemia/reperfusion. Methods: Immunohistochemistry was used to measure the protein expression of MMP9 after cerebral ischemia/reperfusion. Results: MMP9 expression was significantly increased at 20 minutes after ischemia/reperfusion in CA1 in model group〔(42.3±17.1)positive cells〕 compared with shamoperated group〔(4.8±1.6)positive cells〕, P<0.05. In drugintervented group,the MMP9 expression was lower in Yangyin Tongnao granule which was 〔(7.2±2.9)positive cells〕 and nimodipine group which was 〔(12.7±5.3)positive cells〕 than those in model group, all P<0.05. Conclusion: The results demonstrate that the increase of MMP9 expression in CA1 could be induced by cerebral ischemia/reperfusion and MMP9 may be one of the molecular mechanisms of brain damage. Yangyin Tongnao granule are of the prevention and treatment effect on the brain injury induced by cerebral ischemia/reperfusion.

关 键 词：脑缺血 再灌注损伤 MMP9 养阴通脑颗粒 免疫组织化学 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]