Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis柱分配色谱-毛细管区带电泳联用分析板蓝根中五种微量有机酸(英文)  

柱分配色谱-毛细管区带电泳联用分析板蓝根中五种微量有机酸(英文)

作　　者：CHAIYi-fen JISong-gang ZHANGGuo-qing LIUChang-hai 

机构地区：[1]SchoolofPharmacy,SecondMilitaryMedicalUnizersity,Shanghai200433,China [2]The401stHospitalofPLA,Qingdao266071,China [3]DepartmentofPharmacy,EasternInstituteandHospitalofHepatobiliarySurgery,Shanghai200438,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2003年第4期192-195,共4页中国药学（英文版）

基　　金：ScienceandTechnologyDevelopmentFoundationofShanghaiunderGrantNo .0 1DJ190 12

摘　　要：Aim To determine five organic acids in Radix Isatidis . Method The extraction method and the column partition chromatographic conditions were studied. Then a capillary zone electrophoretic method was set up for the determination. Results The linear ranges of quinazolinone acid, n anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5 52-92 0 μg·mL -1 , 5 12-102 μg·mL -1 , 2 28-84 4 μg·mL-1 , 4 78-159 μg·mL -1 , and 1 74-87 0 μg·mL -1 respectively. Conclusion The established method is accurate and simple.Aim To determine five organic acids in Radix lsatidis. Method The extraction method and the column partition chromatographic conditiom were studied. Then a capillary zone dectrophorefic method was set up for the determina-tion. Results The linear ranges of quinazolinone acid, n-anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5.52 - 92.0μg·mL^-1 , 5.12 - 102μg·mL^-1 , 2.28 - 84.4μg·mL^-1, 4.78 - 159 μg·mL^-1, and 1.74- 87.0μg·mL^-1 respectively. Conclusion The established method is accurate and simple.

关 键 词：Radix Isatidis organic acids capillary electrophoresis column partition chromatography 

分 类 号：R284.2[医药卫生—中药学]