Assignments of ~1H and ^(13)C NMR Signals of Mogroside Ⅳa  

Assignments of ^1H and ^13C NMR Signals of Mogroside IVa

作　　者：ZHANGJian-ye YANGXiu-wei 

机构地区：[1]StateKeylaboratoryofNaturalandBiomimeticDrugs,PekingUniversity [2]MedicalandHealthyAnalysisCenter,PekingUniversity,Beijing100083,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2003年第4期196-200,共5页中国药学（英文版）

摘　　要：Aim To investigate the structure of mogroside Ⅳa isolated from traditionalChinese medicine fructus mo-mordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] andsummarize the NMR characteristics of the structure. Methods Common extraction, separation andpurification methods were used. Various NMR techniques including ~1H NMR, ^(13)C NMR, DEPT, ~1H-~1HCOSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidate thestructure. Results ~1H and ^(13)C NMR signals of mogroside Ⅳa were assigned, and spectroscopicbasis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniquesincluding ~1H-~1H COSY, HSQC, HMBC, NOESY spectra are powerful tools for structure analysis. Thestructure determined by NMR methods is identical with energy minimized conformation simulated bycomputer.Aim To investigate the structure of mogroside IVa isolated from traditional Chinese medicine fructus momordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] and summarize the NMR characteristics of the structure. Methods Cormnon extraction, separafion and purification methods were used. Various NMR techniques including ^1H NMR,^13C NMR, DEPT, ^1H-^1H COSY, HSQC, HMBC, NOESY and molecular model simulated by comtmter were used to elucidate the structure. Results ^1H and ^13C NMR signals of mogroside IVa were assigned, and spectroscopic basis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniques including ^1H-^1H COSY, HSQC, HMBC, NOESY spectra are powerful tools for structure analysis. The structure determined by NMR methods is identical with energy minimized conformation simulated by computer.

关 键 词：fructus momordicae mogroside Ⅳa triterpenoid saponin NMR spectrum ASSIGNMENT 

分 类 号：R284[医药卫生—中药学]