重组(CHO细胞)乙型肝炎疫苗稳定性的研究  

Study on Stability of Recombinant CHO Cell HB Vaccine

作　　者：罗璇[1] 蔡岩[1] 官桂范[1] 魏自力[1] 陈万革[1] 金立杰[1] 

机构地区：[1]长春生物制品研究所,吉林长春130062

出　　处：《中国计划免疫》2003年第6期324-325,共2页Chinese Journal of Vaccines and Immunization

摘　　要：为了观察 10 μg/ml、2 0 μg/ml的重组中国仓鼠卵巢细胞 (CHO细胞 )乙型肝炎 (乙肝 )疫苗的稳定性 ,将 10 μg/ml、2 0 μg/ml疫苗放置 4℃ 2、3、4年后 ,分别接种NIH(美国国立卫生研究院 )小鼠 ,采用放射免疫试验 (RIA)检测血清中乙肝病毒表面抗体 (抗 HBs)。根据疫苗的ED50 算出相对效力。 10 μg/ml剂量疫苗相对效力平均值分别为 2年 1 83,3年 1 36 ,4年 0 83;2 0 μg/ml剂量疫苗相对效力平均值分别为 2年 1 91,3年 1 75 ,4年 1 75。结果表明 ,2 0 μg/ml疫苗的稳定性相对优于 10 μg/ml疫苗。To observe and compare the stability of different dosages of recombinant CHO cell hepatitis B vaccine, two kinds of vaccine dosage (10μg/ml, 20μg/ml) were stored at 4℃ for two, three and four years and then were used to inoculate the NIH mice. The serum anti-HBs was detected by radioimmunoassay (RIA). The relative efficacy was calculated by the ED_ 50 . The average relative efficacy of dosage 10μg/ml were 1.83, 1.36 and 0.83, the 20μg/ml were 1.97, 1.75 and 1.75 after storing at 4℃ for 2, 3, 4 years respectively. The results showed the vaccine stability of 20μg/ml is better than that of 10μg/ml.

关 键 词：CHO细胞 乙型肝炎疫苗 稳定性 放射免疫试验 

分 类 号：R512.62[医药卫生—内科学] R392-33[医药卫生—临床医学]