酶联免疫吸附试验定量检测人抗流行性乙型脑炎病毒IgG抗体方法的研究  

Establishment of an ELISA Method for Quantitative Detection of the Antibody against Japanese Encephalitis Virus

作　　者：杨立清[1] 隋洪艳[1] 李红[1] 石男[1] 郭绍红[1] 

机构地区：[1]北京生物制品研究所,北京100024

出　　处：《中国计划免疫》2003年第6期360-361,共2页Chinese Journal of Vaccines and Immunization

摘　　要：为建立快速检测人抗流行性乙型脑炎 (乙脑 )病毒抗体的方法 ,用纯化乙脑病毒选择实验条件 ,通过与蚀斑减少中和试验 (PRNT)的比较 ,确定保护性抗体的酶联免疫吸附试验 (ELISA)的尺度 ,再对两种方法进行比较。结果 :ELISA比PRNT法灵敏度高 16～ 32倍。两种方法有良好的线性关系 (r=0 771,P<0 0 0 1)。PRNT法 1∶10抗体滴度对应的ELISA结果为 0 331U ,两种方法差异无显著的统计学意义 (χ2 =0 14 2 ,P >0 0 5 ) ,总符合率为 81 3%。We developed an ELISA method for guantitative detection of antibody against Japanses encephalitis virus (JEV) and compared it with plaque reduction neutralization test(PRNT).The difference between the two methods was not significant (χ 2=0.142,P>0.05),and also had good linearity(r=0.771).The sensitivity of ELISA was 16-32 times higher than that of PRNT.The agreement with PRNT was 81.3%.

关 键 词：酶联免疫吸附试验 定量检测 人抗流行性乙型脑炎病毒IgG抗体 蚀斑减少中和试验 

分 类 号：R446.61[医药卫生—诊断学]