人胎儿卵巢免疫原性的研究  

The Study of The Immunogenicity in The Human Fetal Ovary

作　　者：刘晓红[1] 郑英[2] 

机构地区：[1]北京市海淀区妇幼保健院产前筛查中心,北京100080 [2]河南医科大学第二附属医院妇产科,河南郑州450003

出　　处：《内蒙古医学杂志》2003年第6期471-474,共4页Inner Mongolia Medical Journal

摘　　要：目的 :比较成人卵巢、胎儿卵巢免疫原性的差异 ,以及培养前后胎儿卵巢免疫原性的变化。方法 :对成人及培养前后胎儿卵巢的冰冻切片用HLA -ABC及HLA -DR单克隆抗体进行免疫组织化学染色 ,观察其阳性细胞形态及分布并计数。结果 :成人卵巢内HLA -ABC及HLA -DR抗原含量显著多于胎儿卵巢 (P <0 0 0 1) ,且培养后胎儿卵巢HLA -DR抗原含量显著低于培养前 (P <0 0 1) ,随培养天数的增加HLA -DR抗原含量逐渐减少 (P <0 0 1)。结论 :胎儿卵巢的免疫原性低于成人卵巢 ,且培养后进一步降低。以培养一段时间的胎儿卵巢作为移植供体 ,必将提高临床卵巢移植的成功率。Objective:To compare the immunogenicity of adult ovary,uncultured and cultured fetal ovary.Methods:To observe the expression of HLA-ABC and HCA-DR antigens by using immunohistochemical method on the frozen sections of adult and fetal ovary.Results:The HLA-ABC and HLA-DR antigens in adult ovary were significantly higher than that in.The HLA-DR antigen in cultured fetal ovary were significantly higher than that in unculturedone,and they decreased following with the increase of cultured days.Conclusions:The immunogenicity of fetal ovary is lower than that of adult ovary,and reduce after being cultured.As a graft of ovary transplantation,the cultured fetal ovary may prolong the survival rate of grafts.

关 键 词：胎儿卵巢 免疫原性 人白细胞抗原 HLA 卵巢移植 免疫组织化学 组织培养 

分 类 号：R339.22[医药卫生—人体生理学] R392.11[医药卫生—基础医学]