饮食诱导肥胖有抵抗性的大鼠解偶联蛋白-2基因的表达  

Expression of uncoupling protein-2 mRNA in diet-induced obesity-resistant rats

作　　者：刘荣[1] 孙长颢[1] 付荣霞[1] 颜跃山 王舒然[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,150001 [2]江苏淮阴快鹿牛奶公司

出　　处：《中华预防医学杂志》2003年第6期446-449,共4页Chinese Journal of Preventive Medicine

摘　　要：目的　探讨高脂饮食诱导肥胖抵抗大鼠褐色脂肪组织 (BAT)、白色脂肪组织 (WAT)和骨骼肌解偶联蛋白 2 (UCP2 )基因的表达。方法　采用 5 0只健康雄性SD大鼠 ,随机分为对照组和高脂组 ,分别用基础饲料和高脂饲料喂养 13周 ,然后根据体重筛选出DIO R和DIO组观察饮食诱导肥胖抵抗 (DIO R)大鼠体重与能量摄入量的变化 ,应用RT PCR方法测定大鼠褐色脂肪组织、白色脂肪组织和骨骼肌UCP2mRNA表达水平。结果　饮食诱导肥胖 (DIO)大鼠体重与总能量摄入量 (4 89 7± 2 0 5 )g、(3436 3± 14 6 5 )kJl明显高于DIO R大鼠 (4 2 5 1± 2 7 1) g ,(316 93± 94 6 )kJ。白色脂肪组织中 ,DIO R大鼠UCP2mRNA的峰面积为 (35 2± 30 )mm2 ,DIO大鼠UCP2mRNA的峰面积为 (10 1±12 )mm2 ;DIO及DIO R大鼠骨骼肌UCP2mRNA的峰面积分别为 (130± 15 )mm2 、(170± 12 )mm2 ;DIO R大鼠和DIO大鼠褐色脂肪组织UCP2mRNA的峰面积为 (12 4± 14 )mm2 、(14 7± 19)mm2 。结论　DIO R大鼠白色脂肪组织UCP2mRNA表达明显增加 ,提示肥胖抵抗与组织特异增加UCP2的表达相关。Objective To explore the expression of uncoupling protein-2 mRNA in brown adipose tissue, white adipose tissue and skeletal muscle of diet-induced obesity-resistant (DIO-R) rats. Methods Fifty male Sprague-Dawley (SD) rats were randomly divided into a control group and a high-fat group and fed with basic diet and high-fat diet respectively for 13 weeks.DIO-R and DIO rats were selected according to their body weight.The change of body weight and the intake of total calorie were observed.Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression of UCP2 mRNA in rat. Results Body weight and total calorie intake in DIO-R rats(425.1±27.1) g,(31 693±946 )k Jwere significantly lower than those in DIO rats(489.7±20.5) g,(34 363±1 465) kJ.The peak area of UCP2 mRNA in white adipose tissue in DIO-R rats was 352±30 and in DIO rats was 101±12.The peak areas of UCP2 mRNA in skeletal muscle in DIO-R and DIO rats were 130±15 and 170±12, respectively.The peak areas of UCP2 mRNA in brown adipose tissue of DIO and DIO-R rats were 124±14 and 147±19, respectively.Conclusion The expression of UCP2 mRNA in white adipose tissue of DIO-R rats increased significantly.These results suggest that obesity-resistance was associated with a tissue-specific increase in UCP2 expression.

关 键 词：饮食 肥胖 大鼠 解偶联蛋白-2 基因表达 褐色脂肪组织 白色脂肪组织 

分 类 号：R589.2[医药卫生—内分泌] R-33[医药卫生—内科学]