单宁酸对棉铃虫谷胱甘肽S-转移酶的影响  

Effects of tannic acid on glutathione S-transferases in Helicoverpa armigera (Hübner)

作　　者：陈凤菊[1] 高希武[1] 雷明庆[1] 郑炳宗[1] 

机构地区：[1]中国农业大学昆虫学系,北京100094

出　　处：《昆虫学报》2003年第6期684-690,共7页Acta Entomologica Sinica

基　　金：国家重大基础研究"973"项目 (G2 0 0 0 0 162 0 7) ;国家自然科学基金项目 ( 3 9770 5 0 5 )

摘　　要：通过培养基混药法 ,研究了植物次生物质单宁酸对棉铃虫Helicoverpaarmigera (H櫣ｂｎｅｒ)谷胱甘肽S 转移酶活性的影响。谷胱甘肽S 转移酶活性随棉铃虫发育期的进程而变化 ,在卵期最低 ,5龄、 6龄幼虫和成虫期最高。用含 0 0 0 5 %单宁酸的饲料饲喂棉铃虫后 ,5龄和 6龄幼虫的谷胱甘肽S 转移酶活性明显降低 ,分别为对照的 5 9%和 6 7%。单宁酸低剂量、短时间处理棉铃虫幼虫 ,可诱导中肠和脂肪体中的谷胱甘肽S 转移酶的活性增加 ,高剂量或低剂量长时间处理没有诱导增加作用 ,甚至还有抑制作用。单宁酸连续处理 4代 ,对棉铃虫 6龄幼虫中肠谷胱甘肽S 转移酶均有抑制作用 ,对脂肪体谷胱甘肽S 转移酶活性无明显影响或有抑制作用。单宁酸处理的第 4代幼虫对溴氰菊酯的敏感度有增加的趋势 。The effects of tannic acid on the specific activity of glutathione S transferases (GSTs) in the cotton bollworm, Helicoverpa armigera (Hübner) fed on artificial diet with tannic acid were investigated. The results showed that the specific activity of GSTs was lowest in the egg stage and highest in the 5th-6th instars of the cotton bollworm. When cotton bollworms were treated with 0 005% tannic acid, the specific activity of GSTs in the 5th and 6th instars was greatly reduced (59% and 67% of the control respectively), while it was induced in the 3rd instar and not different from the control in other developmental stages. The effects of tannic acid on GSTs specific activity were dependent on dosage and time. Low dosage and/or short time of treatment with tannic acid induced high level of GSTs specific activity in both midgut and fat body, while high dosage and/or long time of treatment either depressed GSTs activity or had no effect on it. When cotton bollworms were fed on artificial diet with tannic acid over 4 continuous generations, their GSTs specific activity in the 6th instar was all depressed in midgut, while either depressed or not different from control in fat body. After 4 generations treatment with tannic acid, the larvae of cotton bollworms were more susceptible to deltamethrin, but had no significant difference in susceptibility to parathion methyl.

关 键 词：棉铃虫 植物次生物质 单宁酸 谷胱甘肽S-转移酶 诱导 

分 类 号：Q965.9[生物学—昆虫学]