有机碳源对产EPA微藻(Nannochloropsis sp.)生长及光合作用的影响  

Effect of Organic Carbon Sources on Growth and Photosynthesis of Nannochloropsis sp.

作　　者：徐芳[1] 胡晗华[1] 丛威[1] 蔡昭铃[1] 欧阳藩[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《过程工程学报》2003年第6期560-563,共4页The Chinese Journal of Process Engineering

摘　　要：研究了几种有机碳源对Nannochloropsis sp.生长及光合作用的影响. 结果表明,Nannochloropsis sp.具有混合营养生长的能力,葡萄糖对其生长有明显的促进作用,乙醇对藻细胞的生长有一定的促进作用,乙酸钠不能促进其生长,而乙酸、草酸、甘氨酸则抑制其生长. 在含30 mmol/L葡萄糖的培养基中培养8 d后,该藻细胞生长的最大生物量干重可达550 mg/L,是光自养培养条件下(最大生物量干重为392 mg/L)的1.4倍. 通过测定该藻细胞的光合放氧速率发现,该藻细胞在自养和混养条件下,最大净光合放氧速率几乎不变,混养条件下的暗呼吸速率、光补偿点高于自养条件,而光饱和点低于自养条件.The effect of some organic carbon sources on the growth and photosynthesis of Nannochloropsis sp. were investigated. The results showed that Nannochloropsis sp. could grow mixotrophically. Glucose could promote the growth of Nannochloropsis sp. efficiently. Ethanol could increase its growth to a certain extent. Sodium acetate had no effect on its growth, but acetic acid, oxalic acid and glycine inhibited its growth. After 8-d culture, the cell concentration in the medium with 30 mmol/L glucose reached 550 mg/L (dry weight), which was 1.4-fold of that obtained under photoautotrophic condition. The photosynthetic parameters of Nannochloropsis sp. under the mixotrophic and photoautotrophic cultivations were also studied. The net maximum photosynthetic rate was nearly equal under the mixotrophic and photoautotrophic conditions. The respiration rate, initial slope and compensation irradiance of Nannochloropsis sp. in mixotrophic culture were higher than those in photoautotrophic culture, while the saturation irradiance in mixotrophic culture was lower than that in photoautotrophic culture.

关 键 词：有机碳源 Nannochloropsissp. EPA微藻 混合营养培养 光自养培养 

分 类 号：Q949.2[生物学—植物学]