糖尿病小鼠一氧化氮与一氧化氮合酶活性测定结果分析  

Analysis of detection results of NO and NOS activity in mice with DM

作　　者：张宸豪[1] 田晶[2] 赵大力[1] 李民飞[1] 李平[2] 马爱新[1] 

机构地区：[1]第四军医大学吉林军医学院，吉林吉林132013 [2]第四军医大学吉林军医学院生理学教研室，吉林吉林132013

出　　处：《第四军医大学吉林军医学院学报（86813X）》2003年第4期194-195,198,共3页Journal of Jilin Military Medical College Fourth Military Medical University

摘　　要：目的 观察Ⅰ型糖尿病(DM)小鼠模型血清一氧化氮(NO)与组织一氧化氮合酶(NOS)的变化，探讨NO在胰岛细胞中损伤机制。方法 用柯萨奇B4病毒(CB4V)感染Swiss小鼠建立DM模型，应用放射免疫法，检测其血清NO与组织NOS活性，并分析两者之间的关系。结果：DM模型小鼠血清NO含量随血糖升高和胰岛素水平下降而升高，组织NOS活性随血清NO含量升高而增强，且两者呈正相关(r=0．8175，P<0．01)。结论 NO可能是造成胰岛B细胞损伤的主要因素。Objective To observe the change of NO and NOS in serum of mouse models with I-DM and investigate the injury mechanism of NO in islet cells.Methods CB 4V was used to infect Swiss mice for preparation of DM models and then radioimmunity methods was applied in detection of NO and NOS activity in serum and finally their relation was analyzed.Results The NO content in serum of DM model mouse increased with the increase of blood sugar and decrease of insulin level while NOS activity would be improved with in...

关 键 词：糖尿病 小鼠 一氧化氮合酶 NOS 病毒感染 胰岛细胞 DM 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]