利用cDNA-AFLP技术鉴定甘蓝显性核不育基因相关表达序列  

Gene Expression Associated with Dominant Male Sterility in Cabbage is Revealed by cDNA-Amplified Fragment Length Polymorphism Analysis

作　　者：娄平[1] 王晓武[1] Guusje.Bonnema 方智远[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]Laboratory of Plant Breeding,Wageningen University,Wageningen 6700AJ,Netherlards

出　　处：《园艺学报》2003年第6期668-672,共5页Acta Horticulturae Sinica

基　　金：农业部蔬菜遗传与生理重点实验室项目;中-荷联合园艺作物基因组技术实验室资助项目

摘　　要：利用cDNA-AFLP技术,比较分析了一个甘蓝与两个青花菜自交系回交转育的显性核基因雄性不育材料与对应可育亲本植株花蕾发育过程中基因表达的差异。将花蕾混合提取RNA合成cDNA建立6个cDNA池,分析128个引物组合获得了26000个片段,共鉴定24个片段与雄性不育相关,其中13条表现为质的差异,11条表现为量的差异;将其中引物组合A16T15产生的300 bp左右的差异片段进行克隆测序,BLAST结果表明,该序列与花粉特异性相关的硫氧还蛋白氨基酸序列同源性达89％。cDNA-AFLP was used to detect differentially expressed genes during flower bud development of male sterile and fertile lines of cabbage and broccoli. Expression profiles were generated around 26,000 cDNA fragments (128 primer combinations). Transcriptional changes were observed for 24 cDNA fragments, from which, 13 were only expressed in fertile buds, while the other 11 were upregulated in fertile buds. Sequencing of a 300 bp differential fragment amplified by primer combination A16T15 revealed 89% similarity to the gene thioredoxin, which is related to pollen development in the Brassica family.

关 键 词：CDNA-AFLP 技术鉴定 甘蓝 显性核不育基因 基因表达 基因序列 

分 类 号：S635[农业科学—蔬菜学]