黄瓜单体异附加系的筛选与观察  

Production and Characterization of Monosomic Alien Addition Lines in Cucumber

作　　者：陈劲枫[1] 罗向东[1] 钱春桃[1] 曹清河[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室

出　　处：《园艺学报》2003年第6期725-727,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30170644);国家高技术研究发展计划专项经费项目(2001AA241123;2002AA241251.2002AA207012);教育部跨世纪优秀人才培养计划;教育部科技研究重点项目(01097)

摘　　要：将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交,经胚胎拯救获得了两个2n:15(14C+1H)的单体异附加系,编号分别为02-17和02-39。02-17植株叶形为戟形,02-39为深陷的掌状形,并且果刺均为白色,不同于异源三倍体的掌状心脏形和黑刺,果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现,单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成,出现三价体的频率为4％-5％,中期Ⅱ不均等分裂形成不同的两极,一极染色体为8条,另一极为7条。RAPD分析证明它们分别附加了野生种Cucumis hystrix的染色体。Two monosomic alien addition lines 02-17 and 02-39 (2n=15, 14C+1H) were identified among 52 plants derived from embryo rescue of an Cucumis allotriploid x cucumber. Different from their allotriploid parent, the plants have white spine, and the shape of leaves in 02-17 and 02-39 was hastate and palmate, respectively. In pollen mother cells (PMCs) of 02-17 and 02-39, 7 bivalents and 1 univalent were observed at diakinesis and metaphase Ⅰ. Low frequency (about 4% - 5%) of trivalents has also been observed at metaphase Ⅱ . At metaphase Ⅱ , 8 chromosomes went to one pole while 7 to another. RAPD analysis indicated these two plants were added with one alien chromosome from C. hystrix .

关 键 词：黄瓜 单体异附加系 筛选技术 异源三倍体 '北京截头’ 杂交 

分 类 号：S642.2[农业科学—蔬菜学]